FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_39.35100000045415.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_39.35100000045415.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333293
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC103963.1191774204678766No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGT16980.5094616448590279No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCG14150.42455137071585664No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG8000.2400290435142652No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA6480.19442352524655485No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG4900.14701778915248745No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT4850.1455176076305233No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA4170.12511513893181075No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA3910.11731419501759713No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT3870.11611404980002579No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC3710.11131346892974049No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT3470.10411259762431253No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC3470.10411259762431253No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC11400.041.6427945
ATGCCGT12800.036.9123444
GACCCGA250.002105449335.9983211
TATGCCG14300.033.04041743
GTATGCC14300.032.8830842
CGTATGC14550.032.31807741
GGATGTG14800.032.22822630
TCGTATG14550.032.1634540
CTCGTAT14500.032.1191939
GACAGGA14950.031.75437226
CAGGATG15100.031.73692928
AGGATGT15200.031.52813329
AAAAAGT8000.031.5174451
GATCTCG14900.031.40793236
ACAGGAT15200.031.38011427
GTGATCT15050.031.2443934
ATCTCGT15000.031.19854437
CGAGACA15300.031.17501623
TCTCGTA14950.031.15239338
GATGTGA15300.031.02796431
Produced by FastQC (version 0.11.2)