FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_37.351000000453d0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_37.351000000453d0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330022
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC107623.260994721564017No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGT37241.12840962117677No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCG22390.6784396191769033No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT7940.24059002127130918No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA6100.18483616243765566No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT5980.1812000412093739No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC5420.16423147547739242No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT5060.15332311179254715No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA4970.15059602087133583No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA4890.14817194005248135No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTC4600.13938464708413378No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT4400.13332444503699753No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTAT4260.12908230360400216No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTATA4250.12877929350164535No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC3930.11908297022622735No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT3670.11120470756495023No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT3530.10696256613195484No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10950.044.17436245
GCCCCTA504.858157E-836.00242227
TCGCCCC551.1243901E-732.72451825
AAAAAGT13800.032.3141631
ACTATCG352.8174417E-432.1401511
ATGCCGT15150.031.92800544
AAAAGTA15200.030.6481552
CTAGATG602.4055225E-730.0065674
TAATGTG300.00513650230.0065675
AATACAT300.00513650230.0065673
CCCTATA602.408724E-730.0020229
GGATCCG300.005140310630.0020232
GTAGGCA300.00514412129.9974758
AGTCGGC300.00514412129.99747520
GTTCGAT702.8754584E-828.92613411
TATGCCG17000.028.45348743
GTATGCC17150.028.20462242
CCTATCC406.148876E-428.1311593
CCCCTAT654.852809E-727.69417228
CGCCCCT654.852809E-727.69417226
Produced by FastQC (version 0.11.2)