FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_36.351000000453b6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_36.351000000453b6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377689
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC72321.9148029198626384No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGT18670.4943220480342292No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCG11490.30421855018282234No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT8020.21234401849140433No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC6530.1728935711656945No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT6460.17104019444569474No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA6220.16468575997712404No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT6050.16018470222855313No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA5290.14006232641141256No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTAT4510.11941041438855778No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT4430.11729226956570088No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT4280.11332074802284418No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTC4080.10802538596570194No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA3910.10352432821713103No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCTC3870.10246525580570258No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC3790.10034711098284568No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8250.042.27086345
ATGCCGT10650.032.74503744
AAAAAGT13250.030.9124811
ACGGTAA300.00514404429.99867839
AAAAGTA14350.029.4799542
TATGCCG12100.028.82104343
CGTATGC12400.028.12375841
GTATGCC12400.028.12375842
TCGTATG12600.027.67735140
ACAGGAT12850.027.66414827
GGATGTG12950.027.45415930
GATCTCG12650.027.39009536
CTCGTAT12700.027.2822639
TCTCGTA12900.027.03369138
AGACAGG13250.026.99880825
GGTTCTA507.148039E-526.99880827
GACAGGA13150.026.86193326
CCCACGA13250.026.82900419
CGAGACA13300.026.72814623
CAGGATG13450.026.6008628
Produced by FastQC (version 0.11.2)