FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_35.351000000453aa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_35.351000000453aa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282241
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC44081.561785849681655No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGT9380.3323400923324393No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC3390.12011011865745941No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA3060.10841798321292795No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCG3000.10629214040483133No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTCC253.8846563E-544.99734513
TGCCGTC5450.041.69478645
CGTTCCC301.1381364E-437.49778414
ATGCCGT6350.035.7852944
GATCTCG6650.033.8325936
TATGCCG6750.033.6646843
GTATGCC6800.033.4171442
CGTATGC6850.033.17322541
ATCTCGT6850.032.84477637
CTCGTAT6850.032.84477639
GGATGTG6900.032.6125530
TCTCGTA6900.032.6067738
GACAGGA7000.032.14665626
AGACAGG7000.032.14665625
ACGAGAC7000.032.14665622
CACGAGA7050.031.91866521
CAGGATG7050.031.91866528
CGAGACA7000.031.8251923
ACAGGAT7100.031.69388627
CCACGAG7100.031.68827220
Produced by FastQC (version 0.11.2)