FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_33.3510000004535a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_33.3510000004535a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134031
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC82546.158276816557364No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGT17121.2773164417187068No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCG6090.4543724959151241No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1870.1395199617998821No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1590.1186292723325201No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1470.10967611970365064No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1420.10594563944162172No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGACG206.9998007E-445.014565
TGACCAC207.012691E-444.9977636
TTGACCA207.012691E-444.9977635
CCCATAA207.012691E-444.9977624
TGCCGTC9750.044.0747345
TAGACGG250.002097152236.0116466
GGGTATA250.002097152236.0116461
ACATAAT250.002097152236.0116461
CTTCGTG250.002100999735.99820725
TGCCTCT250.002100999735.99820720
CATCGAT250.002100999735.99820718
ATTGACC250.002100999735.99820734
ATGCCGT11950.035.96055244
TATGCCG12650.033.97064243
ATCTCGT12650.033.79278637
CGTATGC12750.033.7042141
CTCGTAT12750.033.7042139
TCTCGTA12700.033.6597438
GTATGCC12800.033.57254842
GTGATCT12750.033.52774434