FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_32.35100000045330.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_32.35100000045330.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences232873
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC39641.7022153706097314No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGT10660.4577602384132124No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT2840.1219548852808183No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA2590.11121942002722514No Hit
CCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATG2580.1107900014170814No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4950.037.7215145
ATGCCGT5800.032.5812344
GTATGCC5900.032.41030542
CATCTCG6050.031.61353336
CCCACGA6050.031.61353319
ACTTGGC1000.031.4951928
GCCCACG6100.031.35440318
CGTATGC6100.031.35440341
CTCGTAT6100.031.35440339
TATGCCG6150.031.09281243
TCGTATG6250.030.601940
CGAGACC6150.030.36773723
CACGAGA6300.030.35902821
TCTCGTA6300.030.35902838
ATCAGAC6200.030.12283530
CACCCTT300.005137370430.00186326
CCACGAG6500.029.77107820
ACGAGAC6450.029.65300422
ATCTCGT6600.028.97906937
GAGACCA6450.028.95528624
Produced by FastQC (version 0.11.2)