FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_30.351000000452fb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_30.351000000452fb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271165
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC101673.7493776851732337No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGT25970.9577194696955729No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCG4180.15414968745966479No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC11200.042.98948345
ATGCCGT13900.034.6390144
GTATGCC14350.033.70955742
CGTATGC14500.033.3608441
TATGCCG14500.033.3608443
TCGTATG14500.033.05050740
GACATCT14300.033.0407434
CATCTCG14550.032.6276636
CTCGTAT14750.032.3377939
CCCACGA14800.032.22854219
TCTCGTA14850.031.96851738
AGACATC14800.031.924533
GCCCACG14950.031.90517818
ACGAGAC15000.031.79882622
CGAGACC14900.031.7102423
CCATCAG15050.031.69318228
ATCTCGT15000.031.64883237
CCACGAG15150.031.48398820
CAGACAT15100.031.43923632
GACCATC15400.031.11898226