Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_0113_pc_3.35100000044fa1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 33986 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC | 9719 | 28.597069381510032 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT | 728 | 2.1420584946742776 | RNA PCR Primer, Index 44 (95% over 21bp) |
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT | 327 | 0.962160889778144 | RNA PCR Primer, Index 44 (95% over 23bp) |
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG | 237 | 0.6973459659859942 | No Hit |
ATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTTC | 190 | 0.5590537280056495 | RNA PCR Primer, Index 37 (95% over 23bp) |
TATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTT | 98 | 0.28835402812922967 | RNA PCR Primer, Index 37 (95% over 22bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 1055 | 0.0 | 40.7346 | 45 |
AGCCCAC | 1145 | 0.0 | 37.72926 | 17 |
CCACGAG | 1145 | 0.0 | 37.72926 | 20 |
GCCCACG | 1150 | 0.0 | 37.565216 | 18 |
GAGCCCA | 1150 | 0.0 | 37.565216 | 16 |
CACGAGA | 1150 | 0.0 | 37.565216 | 21 |
CCCACGA | 1150 | 0.0 | 37.565216 | 19 |
ACGAGAC | 1150 | 0.0 | 37.565216 | 22 |
CGAGCCC | 1155 | 0.0 | 37.402596 | 15 |
AGACCCT | 1155 | 0.0 | 37.402596 | 25 |
TCCGAGC | 1155 | 0.0 | 37.402596 | 13 |
CCTAGAA | 1155 | 0.0 | 37.402596 | 29 |
CCCTAGA | 1155 | 0.0 | 37.402596 | 28 |
ATGCCGT | 1150 | 0.0 | 37.369564 | 44 |
TAGAATA | 1150 | 0.0 | 37.369564 | 31 |
TCTCGTA | 1145 | 0.0 | 37.336246 | 38 |
TATCTCG | 1140 | 0.0 | 37.30263 | 36 |
ATATCTC | 1140 | 0.0 | 37.30263 | 35 |
ACCCTAG | 1160 | 0.0 | 37.24138 | 27 |
CCGAGCC | 1160 | 0.0 | 37.24138 | 14 |