FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_3.35100000044fa1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_3.35100000044fa1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33986
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC971928.597069381510032No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT7282.1420584946742776RNA PCR Primer, Index 44 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT3270.962160889778144RNA PCR Primer, Index 44 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG2370.6973459659859942No Hit
ATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTTC1900.5590537280056495RNA PCR Primer, Index 37 (95% over 23bp)
TATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTT980.28835402812922967RNA PCR Primer, Index 37 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10550.040.734645
AGCCCAC11450.037.7292617
CCACGAG11450.037.7292620
GCCCACG11500.037.56521618
GAGCCCA11500.037.56521616
CACGAGA11500.037.56521621
CCCACGA11500.037.56521619
ACGAGAC11500.037.56521622
CGAGCCC11550.037.40259615
AGACCCT11550.037.40259625
TCCGAGC11550.037.40259613
CCTAGAA11550.037.40259629
CCCTAGA11550.037.40259628
ATGCCGT11500.037.36956444
TAGAATA11500.037.36956431
TCTCGTA11450.037.33624638
TATCTCG11400.037.3026336
ATATCTC11400.037.3026335
ACCCTAG11600.037.2413827
CCGAGCC11600.037.2413814