FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_29.351000000452d1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_29.351000000452d1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333668
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC177055.306172602706882No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGT46621.397197213997147No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCG10020.30029850030569305No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC17750.043.997245
ATGCCGT22750.034.32748444
TATGCCG23850.032.82876643
GTATGCC24050.032.4622142
CTCGTAT23850.032.45142439
CGTATGC23950.032.4098741
CATCTCG23900.032.289436
TCGTATG24050.032.08800540
ATCTCGT24350.031.8774737
TCTCGTA24350.031.7850738
GACATCT24550.031.70942334
CGAGACC24900.031.62513523
CAGACAT24750.031.5440932
ACGAGAC25200.031.33792922
AGACATC25000.031.31864433
CCCACGA25200.031.15936519
TCAGACA25100.031.1042331
GAGACCA25250.031.09766424
GCCCACG25300.031.03620518
ATCAGAC25250.031.0085630
Produced by FastQC (version 0.11.2)