FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_28.351000000452b7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_28.351000000452b7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338352
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC108873.217654986522911No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGT23350.6901097082328462No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCG5120.15132170047760912No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC13550.043.00625245
CCGATCA250.002105284235.9990549
ATGCCGT16400.035.53260444
TATGCCG16550.035.21055643
CGTATGC16950.034.64510741
GTATGCC16900.034.48134242
TCGTATG17050.034.30994840
CCCACGA17200.034.27235419
CGAGACC17200.034.27235423
GACCATC17150.034.2410826
ACGAGAC17150.034.2410822
CATCTCG17100.034.20962536
GCCCACG17250.034.17301618
CTCGTAT17150.034.1098939
CACGAGA17250.034.04258721
CCACGAG17450.033.6524120
CAGACAT17450.033.6524132
TCTCGTA17400.033.6198138
ATCAGAC17550.033.4606630
GAGACCA17850.033.02434524
Produced by FastQC (version 0.11.2)