FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_27.3510000004529e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_27.3510000004529e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287131
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC105843.6861223622667008No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGT18360.639429389372795No Hit
GTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA6840.23821879211927657No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT6390.22254650316406102No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG5980.20826730656042014No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC5020.17483309012262696No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCT4930.17169863233158386No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC4930.17169863233158386No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA4230.14731951617902628No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCTC4070.14174714677272743No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAA4050.14105060059694008No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC3960.13791614280589695No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATC3790.13199550031170443No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3730.12990586178434235No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGCTGTCTCTTATACACATCTCCG3730.12990586178434235No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC3700.1288610425206613No Hit
TACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTC3650.1271196770811929No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA3490.12154730767489405No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA3490.12154730767489405No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC3270.1138852997412331No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT3240.11284048047755205No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC3130.10900947651072158No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCG2980.1037853801923164No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATC2970.10343710710442272No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10650.043.30801445
ATGCCGT12850.036.06850444
CATCTCG13400.034.42017736
TATGCCG13400.034.42017743
ATCAGAC13200.034.26587330
CTCGTAT13500.034.16521539
GTATGCC13500.034.16521542
CGTATGC13550.034.03914341
GAGACCA13600.033.91399824
AGACCAT13550.033.7129225
GACATCT13550.033.7129234
GACCATC13600.033.5889826
CGAGACC13800.033.58552623
CCACGAG13800.033.58552620
CATCAGA13500.033.5044129
CAGACAT13650.033.46594232
AGACATC13700.033.34380333
ATCTCGT13850.033.3018337
TCGTATG13850.033.3018340
TCAGACA13800.032.93911731