FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_26.35100000045274.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_26.35100000045274.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311158
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC100063.215729629320153No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGT30950.9946715173641686No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCG4580.14719210176180592No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT3350.10766234517512004No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGCT207.027079E-444.9977511
CGCGCTT207.027079E-444.9977512
CGCGCGC207.027079E-444.9977510
TCGCGCG207.027079E-444.997759
GGGGCTA207.027079E-444.9977536
TGCCGTC10800.042.9145245
GTCGCGC250.002105269335.99828
GGGCTAG250.002105269335.998237
GGTCGCG250.002105269335.99827
GCGCTTA250.002105269335.998213
ATGCCGT13750.033.7074144
TATGCCG14500.031.96391743
GACTCTC855.456968E-1231.7631197
GTCCTAA501.9663585E-631.5186841
GTATGCC14750.031.42215742
CATCTCG14750.031.11708836
CAGACAT14950.030.85130132
GACATCT14850.030.75603934
ATCTCGT15000.030.74846337
CGAGACC14950.030.70080823
Produced by FastQC (version 0.11.2)