FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_25.3510000004525b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_25.3510000004525b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120515
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC1588913.184250923121605No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGT29672.461934199062357No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCG4840.4016097581213957No Hit
TTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTCT4040.3352279799195121No Hit
ATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTCTTC1610.1335933286312907Illumina PCR Primer Index 3 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGTG207.008148E-445.045
GACGTAT207.008148E-445.036
CCGTCTT253.8699836E-545.044
CACTCCT207.008148E-445.011
CACTCAG207.008148E-445.038
TTCGGGT253.8699836E-545.017
CTCCTTG207.008148E-445.013
CCTATAC207.008148E-445.03
GATAGGG207.008148E-445.07
TGCCGTC17950.042.8690845
ATGCCGT20850.036.90647544
CACGAGA21350.036.04215221
GAGGAGT250.002099648136.045
CAGAACT250.002099648136.023
CCCTATA250.002099648136.02
GACACTC250.002099648136.036
CGGTTCT250.002099648136.014
GAGAGAC250.002099648136.032
GCTGAGG250.002099648136.042
CCCACGA21250.036.019
Produced by FastQC (version 0.11.2)