FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_24.35100000045231.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_24.35100000045231.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47000
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC3270.6957446808510639RNA PCR Primer, Index 23 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC403.3648394E-739.37500441
CTCGTAT403.3648394E-739.37500439
TATGCCG403.3648394E-739.37500443
CCCACGA403.3648394E-739.37500419
GTATGCC403.3648394E-739.37500442
TCGTATG403.3648394E-739.37500440
GCCCACG458.4663407E-735.018
GAGAGTT458.4663407E-735.029
TCTCGTA458.4663407E-735.038
CCGAGCC458.4663407E-735.014
CGAGCCC458.4663407E-735.015
CGAGACG401.5290803E-533.75000423
AGCCCAC501.9287145E-631.517
AGACGAG501.9287145E-631.525
ATGCCGT501.9287145E-631.544
TGCCGTC501.9287145E-631.545
GAGACGA453.420873E-530.024
GAGTTGA554.05438E-628.63636231
ACGAGAC554.05438E-628.63636222
CCACGAG554.05438E-628.63636220
Produced by FastQC (version 0.11.2)