FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_23.35100000045217.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_23.35100000045217.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198912
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC127776.423443532818533RNA PCR Primer, Index 23 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGT24001.2065637065637065No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCG6030.3031491312741313No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC15000.043.3444345
GGCAGTG301.1373911E-437.4951819
GGGCAGT301.1373911E-437.4951818
ATGCCGT17800.036.52620344
AACTGGG250.002104257535.9953777
TATGCCG18350.035.55401643
GCCCACG18500.035.5089518
TCGTATG18200.035.4762140
GTATGCC18400.035.457442
CCCACGA18550.035.41324219
CCACGAG18500.035.38734420
CGTATGC18450.035.3613141
GATCTCG18250.035.25574536
CGAGACG18650.035.22335423
CACGAGA18800.035.06198521
ACGAGAC18750.035.035522
CTCGTAT18500.035.02252639
CGAGAGT18750.034.9330828
TCTCGTA18600.034.83423238
ACGAGAG18900.034.6558327
Produced by FastQC (version 0.11.2)