FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_22.351000000451fc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_22.351000000451fc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102710
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC19591.9073118488949468RNA PCR Primer, Index 23 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGT3940.3836043228507448No Hit
CCATATGACTTAGCCATTTTCAGTTTGGGATATTGTTATCCAAAGAACCAA1530.14896309999026386No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT1340.1304644143705579No Hit
ACATGGTACCGTACGGAAGATTTAGATAAGAAAGAAGTAACACACTGACGA1310.12754356927270957No Hit
CATTAAAACCGGAAACACACAAACCAATGACTTATTCGAGAAAACACTCAT1300.12656995424009346No Hit
GGATTATGACTGAACGCCTCTAAGTCAGAATCCGGGCTAGAAGCGACGCAT1220.11878103397916463No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA1100.10709765358777139No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA1070.10417680848992308No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1800.040.045
ATAACAG300.005125715429.9999985
CGAGAGT2550.028.23529428
CGAGACG2550.028.23529423
CACGAGA2650.028.01886621
ATGCCGT2600.027.69230744
CGTATGC2650.027.1698141
TATGCCG2650.027.1698143
CCCACGA2650.027.1698119
AGACGAG2650.027.1698125
GTATGCC2650.027.1698142
GCCCACG2750.026.99999818
ACGAGAC2750.026.99999822
CCACGAG2750.026.99999820
GAGAGTT2700.026.66666629
TCGTATG2700.026.66666640
GACGAGA2700.026.66666626
GATCTCG2650.026.32075336
AGCCCAC2850.026.05263117
CGAGCCC2900.025.60344915
Produced by FastQC (version 0.11.2)