FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_21.351000000451d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_21.351000000451d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239879
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC66352.765977847164612RNA PCR Primer, Index 23 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGT22180.9246328357213429No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCG3590.14965878630476198No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCTCG207.0232345E-444.9985437
TGCCGTC7250.043.1365345
GGGAGGT301.13744776E-437.4987837
ATGCCGT8900.035.13931344
TATGCCG9050.034.3082843
CGTATGC9100.034.1197741
GTATGCC9150.033.93332342
TCGTATG9250.033.56648340
CGAGACG9200.033.50434523
GATCTCG9300.033.1440936
CTCGTAT9350.032.96684639
ATCTCGT9350.032.7262137
CGAGAGT9450.032.6179928
ACGAGAC9450.032.6179922
AGAGTTG9650.032.17512530
TCTCGTA9550.032.04084438
ACGAGAG9650.031.9419727
CCCACGA9800.031.91223119
GAGACGA9700.031.7773224
GAGTTGA9750.031.6143631
Produced by FastQC (version 0.11.2)