FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_20.351000000451b8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_20.351000000451b8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331890
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC150464.533429750821055RNA PCR Primer, Index 23 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGT50211.5128506432854258No Hit
CCTTAATCCTGTCCATTACTCCTTGATTCTCTTCTTCCACTGTTTTCACAT9240.2784054957968002No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCG8010.24134502395371962No Hit
GGTGAAGACGGTGAGAAGAAGAAGAAGGAGAAAAAGAAGAAGATTGTTGAA7630.22989544728675163No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT6590.1985597637771551No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC6390.19253367079454037No Hit
GTTTTCACATGATCTCCTTCAACAATCTTCTTCTTTTTCTCCTTCTTCTTC5330.16059537798668233No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCTC5120.15426798035493686No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCT4950.14914580131971436No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC4380.1319714363192624No Hit
GTCCATTACTCCTTGATTCTCTTCTTCCACTGTTTTCACATGATCTCCTTC4280.12895838982795504No Hit
CTTGAAAACCATCTTAAAGCAACTCAAAGCTCAAATCGAAATTTCTAGTTT4230.12745186658230134No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC4180.12594534333664767No Hit
GTGTTCTTGTACTCTTCTGCCATACTTAATCTTGATTCGATTGAACACTTA4000.12052185965229444No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA3880.11690620386272559No Hit
TCTTAAAGCAACTCAAAGCTCAAATCGAAATTTCTAGTTTCTCTTTATCAT3700.11148272017837235No Hit
TGTGAAAACAGTGGAAGAAGAGAATCAAGGAGTAATGGACAGGATTAAGGC3700.11148272017837235No Hit
CCTTAATCCTGTCCATTACTCCTTGATTCTCTTCTTCCACTGTTTTCACAC3520.1060592364940191No Hit
ACTTAATCTTGATTCGATTGAACACTTAGCGTGAATGATAAAGAGAAACTA3370.10153966675705807No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT3370.10153966675705807No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTAC207.0164097E-445.012511
CGTTACT207.021622E-445.005732
GGAGCGG207.0268376E-444.9989478
AGCGGGG207.0268376E-444.99894710
GCGGGGA207.0268376E-444.99894711
TGCCGTC15050.043.6534645
TATCTCG250.00210363936.004585
AGGAGCG250.002105195835.9991577
GAGCGGG250.002105195835.9991579
ATGCCGT20600.031.89245644
GCCGTCT501.9763684E-631.49926245
CTCGTAT21400.030.4899439
CGTATGC21650.030.34570941
TATGCCG21650.030.34570943
GTATGCC21650.030.34570942
GATCTCG21450.030.31397436
CGAGACG21800.030.24011823
TCTCGTA21600.030.20762638
ATCTCGT21600.030.10346237
CGAGAGT21900.030.10203428
Produced by FastQC (version 0.11.2)