FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_2.35100000044f87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_2.35100000044f87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25325
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC1300651.35636722606121No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT14085.5597235932872655RNA PCR Primer, Index 44 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG5482.1638696939782824No Hit
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT1700.6712734452122409RNA PCR Primer, Index 44 (95% over 23bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT502.0008883E-1145.05148745
TGCCGTC13950.043.2752645
ATGCCGT15550.038.822544
TCTCCGA16100.037.8410711
TCCGAGC16200.037.74625413
CTCCGAG16150.037.72391512
CCGAGCC16250.037.6301114
CGAGCCC16100.037.561815
AGCCCAC16150.037.44550717
GCCCACG16150.037.44550718
GAGCCCA16150.037.44550716
CCCACGA16150.037.44550719
ATCTCCG16350.037.3999610
TATGCCG16200.037.2648143
ATCTCGT16050.037.25860637
TCTCGTA16050.037.25860638
TATCTCG16050.037.25860636
GTATGCC16150.037.240742
CACGAGA16300.037.17438521
ACGAGAC16300.037.17438522