Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_0113_pc_2.35100000044f87.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 25325 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 50 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC | 13006 | 51.35636722606121 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT | 1408 | 5.5597235932872655 | RNA PCR Primer, Index 44 (95% over 21bp) |
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG | 548 | 2.1638696939782824 | No Hit |
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT | 170 | 0.6712734452122409 | RNA PCR Primer, Index 44 (95% over 23bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCCGTCT | 50 | 2.0008883E-11 | 45.051487 | 45 |
TGCCGTC | 1395 | 0.0 | 43.27526 | 45 |
ATGCCGT | 1555 | 0.0 | 38.8225 | 44 |
TCTCCGA | 1610 | 0.0 | 37.84107 | 11 |
TCCGAGC | 1620 | 0.0 | 37.746254 | 13 |
CTCCGAG | 1615 | 0.0 | 37.723915 | 12 |
CCGAGCC | 1625 | 0.0 | 37.63011 | 14 |
CGAGCCC | 1610 | 0.0 | 37.5618 | 15 |
AGCCCAC | 1615 | 0.0 | 37.445507 | 17 |
GCCCACG | 1615 | 0.0 | 37.445507 | 18 |
GAGCCCA | 1615 | 0.0 | 37.445507 | 16 |
CCCACGA | 1615 | 0.0 | 37.445507 | 19 |
ATCTCCG | 1635 | 0.0 | 37.39996 | 10 |
TATGCCG | 1620 | 0.0 | 37.26481 | 43 |
ATCTCGT | 1605 | 0.0 | 37.258606 | 37 |
TCTCGTA | 1605 | 0.0 | 37.258606 | 38 |
TATCTCG | 1605 | 0.0 | 37.258606 | 36 |
GTATGCC | 1615 | 0.0 | 37.2407 | 42 |
CACGAGA | 1630 | 0.0 | 37.174385 | 21 |
ACGAGAC | 1630 | 0.0 | 37.174385 | 22 |