FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_18.35100000045175.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_18.35100000045175.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255153
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC40081.5708222125548201RNA PCR Primer, Index 23 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGT5330.20889427128036905No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5200.039.8054445
TCGCGCG403.4456752E-739.3727729
CTAGTTG356.2316212E-638.56924440
CGTATGC5450.037.979541
GCCCACG5400.037.91451618
TATGCCG5400.037.91451643
CCCACGA5450.037.56667719
GTATGCC5450.037.56667742
ATGCCGT5450.037.56667744
CGAGACG5500.036.81609723
GATCTCG5450.036.7410436
TCGTATG5600.036.5604340
AGACGAG5600.036.15866525
CTCGTAT5800.035.2997339
CGAGAGT5700.035.1295928
ATCTCGT5850.034.9980237
CACGAGA5850.034.9980221
ACGAGAC5850.034.9980222
GTCGCGC458.6685395E-734.9980168
GGTCGCG458.6685395E-734.9980167
Produced by FastQC (version 0.11.2)