FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_17.3510000004515c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_17.3510000004515c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63479
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC1100917.342743269427686RNA PCR Primer, Index 23 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGT14142.2275083098347483No Hit
TTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCT3060.48204918161911814No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCG3050.4804738574961798No Hit
ATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCTTC1380.21739472896548465RNA PCR Primer, Index 23 (95% over 24bp)
CTACTAAAGATACATAACAAGGACACTTTCTGTGTTGGTTAAAGTTTGCAC1050.16540903290852094No Hit
ATGTTAGTACATAGAGTTGCTGCATATTTTGGTTTGGATCATAATATTGAT810.12760125395800187No Hit
GTACAGTGTGATGTTTTGATATACACTACTTCTGTGGATATACACCATCTT810.12760125395800187No Hit
TATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCTT790.12445060571212527RNA PCR Primer, Index 23 (95% over 23bp)
TTCTAATTCTGTGAAGAATCATGGTGGTATTTTGATGGAGATTGCATGAAT780.12287528158918698No Hit
GTGTTAATTTTAGTCGATGAAGGTATGTGGTATTTATGCTCATCTCTTTAA720.11342333685155721No Hit
ATAGAGTTGTTCATAATAGTCTCTGGGGATCTTTTGTATTTCTCTGGGATC660.10397139211392745No Hit
GAAGGATTATGTCAAGTGACATATGATATGTGGAATAAAGGCTGGGACGAA650.10239606799098913No Hit
AATACCATGTTTTGGAACACATATCAGCATTTGAATTTTTCTGCTGAGTTA640.10082074386805084No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGTG302.133389E-644.9992144
TGATATA253.84955E-544.9992117
CACTACT253.84955E-544.9992124
TGCCGTC12600.041.7849845
ACAGTGT356.156237E-638.5707553
ACCATCT301.1279281E-437.49934444
ACACCAT301.1279281E-437.49934442
ACACTAC301.1279281E-437.49934423
TACACTA301.1279281E-437.49934422
TACACCA301.1279281E-437.49934441
TCTCCGA14150.036.88981211
ATGCCGT14300.036.8175444
GATCTCG14250.036.7888336
AGCCCAC14300.036.660217
GAGAGTT14300.036.660229
CGAGAGT14300.036.660228
TGATCTC14300.036.660235
ATCTCCG14250.036.6309410
TCCGAGC14250.036.6309413
AGACGAG14250.036.6309425
Produced by FastQC (version 0.11.2)