FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_16.35100000045132.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_16.35100000045132.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134657
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC113598.435506509130605RNA PCR Primer, Index 45 (95% over 21bp)
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA17981.3352443616002139No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15951.1844909659356737No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT14111.0478474940032825No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13671.0151718811498844No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11650.8651611130501943No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA10060.7470833302390518No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA9810.728517641117803No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8690.6453433538546083No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA6360.4723111312445695No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6310.4685979934203198No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6180.4589438350772704No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5020.37279903755467597No Hit
GTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA4410.3274987560988289No Hit
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT4330.32155773558002926No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3950.2933378881157311No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3940.29259526055088114No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3910.29036737785633127No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA3470.2576917650029334No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG3320.2465523515301841No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA3300.24506709640048419No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA3240.2406113310113845No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA3040.22575877971438546No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA2430.18045849825853835No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2260.16783382965608917No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT2220.16486331939668936No Hit
ATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCTTC2200.16337806426698945RNA PCR Primer, Index 45 (95% over 24bp)
GGTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2160.16040755400758966No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA1970.14629763027544057No Hit
GAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1890.14035660975664097No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA1870.13887135462694106No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT1800.13367296167299136No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT1760.13070245141359157No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA1670.124018803329942No Hit
GTATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA1660.12327617576509205No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1580.11733515524629244No Hit
GTACGGGAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1540.11436464498689262No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT1430.10619574177354314No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAA1420.10545311420869319No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA1360.10099734881959348No Hit
GTATCAACGCAGAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA1360.10099734881959348No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC12400.041.3760945
ATGCCGT13550.037.86446844
CTCGTAT13900.036.73550839
TCTCGTA13850.036.7057138
ATTAATC13950.036.61743533
AATCTCG13950.036.6038436
TCATTAA13900.036.58726531
TTCATTA13850.036.5568730
TTAATCT14050.036.5169834
CATTAAT14000.036.4866632
TAATCTC14000.036.4866635
ATCTCGT14000.036.4731137
GTATGCC14000.036.4731142
CGTATGC14050.036.34331541
TCGTATG14050.036.34331540
AGACGTT14000.036.32592825
ACGTTCA13950.036.29481527
CGTTCAT13950.036.29481528
CGAGACG14200.036.28970323
GACGTTC14000.036.1651926
Produced by FastQC (version 0.11.2)