FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_15.35100000045125.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_15.35100000045125.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263626
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC5956722.595267538103222RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT2835210.754629664752338No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG26841.0181089877326213No Hit
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT13240.5022266392540948No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA4970.18852465234840265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA4310.16348918543694477No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC3980.1509714519812158No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC3970.1505921267249816No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC3770.1430056216002974No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC3520.13352249019444212No Hit
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG3170.12024610622624475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTCTGTCTCTTATACACATCTCCGAG2960.11228027584532634No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTCT406.7866495E-944.996216
ATAGACA302.16037E-644.9962044
TGCCGTC65850.043.5695145
TCCAATG250.00210308236.0037924
GCTACAG250.00210308236.0037935
CGGGCAA250.002105041435.99696417
AACCTAG250.002105041435.9969647
GCCGTCT2150.035.58514445
TAGGGTC401.5569238E-533.7471585
GTCCAAT352.812781E-432.14624423
AAGCTAC352.812781E-432.14624433
CTATATG1450.031.0318682
TCTATAT1550.030.4870851
GTAAAGC300.005137498530.00316430
CTCGGCA300.00514226829.9974716
AGTTCCA300.00514226829.9974719
AGATGCA300.00514226829.9974710
ATGCCGT96550.029.6924144
TATGCCG100350.028.52318443
TCGTATG99950.028.49684740
Produced by FastQC (version 0.11.2)