FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_14.351000000450fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_14.351000000450fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371654
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC84622.276848897092457RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT19700.5300629079735453No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT5740.15444472547046445No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA5700.1533684556065588No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA4890.13157399086246885No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC9500.043.81107345
ATGCCGT11800.035.27162644
CGTATGC12000.034.4962941
GTATGCC12000.034.4962942
TATGCCG12150.034.2555743
CTCGTAT12050.033.7930439
TCGTATG12050.033.7930440
GACGTTC12200.033.75090826
CACGAGA12200.033.75090821
CGAGACG12300.033.65944323
ACGTTCA12350.033.34097727
ACGAGAC12350.033.34097722
TCTCGTA12250.033.24131838
ATCTCGT12300.033.10619437
CGTTCAT12450.033.07317728
CCCACGA12500.032.93645519
AGACGTT12600.032.8580325
ATTAATC12550.032.80964733
AATCTCG12400.032.65777636
CCACGAG12750.032.2906420