Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_0113_pc_14.351000000450fd.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 371654 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 40 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC | 8462 | 2.276848897092457 | RNA PCR Primer, Index 45 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT | 1970 | 0.5300629079735453 | No Hit |
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT | 574 | 0.15444472547046445 | No Hit |
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA | 570 | 0.1533684556065588 | No Hit |
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA | 489 | 0.13157399086246885 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 950 | 0.0 | 43.811073 | 45 |
ATGCCGT | 1180 | 0.0 | 35.271626 | 44 |
CGTATGC | 1200 | 0.0 | 34.49629 | 41 |
GTATGCC | 1200 | 0.0 | 34.49629 | 42 |
TATGCCG | 1215 | 0.0 | 34.25557 | 43 |
CTCGTAT | 1205 | 0.0 | 33.79304 | 39 |
TCGTATG | 1205 | 0.0 | 33.79304 | 40 |
GACGTTC | 1220 | 0.0 | 33.750908 | 26 |
CACGAGA | 1220 | 0.0 | 33.750908 | 21 |
CGAGACG | 1230 | 0.0 | 33.659443 | 23 |
ACGTTCA | 1235 | 0.0 | 33.340977 | 27 |
ACGAGAC | 1235 | 0.0 | 33.340977 | 22 |
TCTCGTA | 1225 | 0.0 | 33.241318 | 38 |
ATCTCGT | 1230 | 0.0 | 33.106194 | 37 |
CGTTCAT | 1245 | 0.0 | 33.073177 | 28 |
CCCACGA | 1250 | 0.0 | 32.936455 | 19 |
AGACGTT | 1260 | 0.0 | 32.85803 | 25 |
ATTAATC | 1255 | 0.0 | 32.809647 | 33 |
AATCTCG | 1240 | 0.0 | 32.657776 | 36 |
CCACGAG | 1275 | 0.0 | 32.29064 | 20 |