FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_13.351000000450d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_13.351000000450d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392318
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC120683.076076040355018RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT57011.4531578974199502No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG8180.20850432557262222No Hit
CCTTAATCCTGTCCATTACTCCTTGATTCTCTTCTTCCACTGTTTTCACAT5490.13993749968138092No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC12850.043.77233545
GGCAGTT300.00514431429.9986419
TGGCAGT300.00514431429.9986418
CGGGACT300.00514431429.9986416
ATGCCGT19100.029.44892944
ACTGGCC406.1604806E-428.1237248
TATGCCG20100.027.8718743
GTATGCC20250.027.66541342
CGAGACG20400.027.57227923
CGTATGC20400.027.35170241
CTCGTAT20500.027.21827739
GAGACGT20550.027.15205424
TCGTATG20550.027.15205440
GACGTTC20500.027.10852626
AGACGTT20750.027.10720625
TCATTAA20800.027.04204431
CGTTCAT20650.027.02056728
AATCTCG20900.027.02030636
GCAACCC507.148793E-526.9987758
CCCACGA20950.026.9558219
Produced by FastQC (version 0.11.2)