FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_12.351000000450b9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_12.351000000450b9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414513
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC133593.2228181022066855RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT36960.891648754080089No Hit
CCTTAATCCTGTCCATTACTCCTTGATTCTCTTCTTCCACTGTTTTCACAT8930.21543353284456698No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG7770.18744888580092783No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA6730.16235920224456168No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT6060.14619565610728735No Hit
GTTTTCACATGATCTCCTTCAACAATCTTCTTCTTTTTCTCCTTCTTCTTC5220.12593091169637624No Hit
GTCCATTACTCCTTGATTCTCTTCTTCCACTGTTTTCACATGATCTCCTTC4890.11796976210637544No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA4470.10783738990091987No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC14600.042.6852745
CGCGCTA301.1391484E-437.49728812
CTGCGCG301.1391484E-437.4972889
ATGCCGT18450.033.89998644
CGCTAAC401.5589416E-533.7475614
TATGCCG19300.032.40698243
GCCCAAC352.8164888E-432.144413
ACTGCGC352.8184833E-432.140538
TGCGCGC352.8184833E-432.1405310
GCGCTAA352.8184833E-432.1405313
GGGGCTA352.8184833E-432.1405336
CGTATGC19550.031.99256941
CTCGTAT19550.031.87748739
AGACGTT19600.031.80000525
GTATGCC19700.031.7489742
TCTCGTA19650.031.7152638
CGAGACG19650.031.60457823
CACGAGA19750.031.55848521
ATCTCGT19750.031.55467637
TCGTATG19850.031.39571240
Produced by FastQC (version 0.11.2)