FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_11.35100000045090.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_11.35100000045090.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347309
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC103742.986965497582844RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT15780.45435044873585195No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3970.11430743228652296No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3830.11027643971218712No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG3790.10912472754809117No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10200.038.8220445
ATGCCGT11250.035.1986544
CGTATGC11500.034.23781641
CTCGTAT11450.034.19082639
AGACGTT11650.034.1832425
TATGCCG11600.034.1366243
ATTAATC11550.034.089633
GACGTTC11700.033.84485626
CGTTCAT11650.033.7969928
CGAGACG11650.033.7969923
TTCATTA11750.033.70083630
TCTCGTA11650.033.60386338
CATTAAT11750.033.50935432
GTATGCC11750.033.50935442
TCGTATG11750.033.50935440
AATCTCG11750.033.3178736
GTTCATT11900.033.08696729
ATCTCGT11850.033.03670537
TCATTAA12000.032.99873431
ACGTTCA11950.032.94852427