Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_0113_pc_11.35100000045090.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 347309 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC | 10374 | 2.986965497582844 | RNA PCR Primer, Index 45 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT | 1578 | 0.45435044873585195 | No Hit |
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA | 397 | 0.11430743228652296 | No Hit |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 383 | 0.11027643971218712 | No Hit |
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG | 379 | 0.10912472754809117 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 1020 | 0.0 | 38.82204 | 45 |
ATGCCGT | 1125 | 0.0 | 35.19865 | 44 |
CGTATGC | 1150 | 0.0 | 34.237816 | 41 |
CTCGTAT | 1145 | 0.0 | 34.190826 | 39 |
AGACGTT | 1165 | 0.0 | 34.18324 | 25 |
TATGCCG | 1160 | 0.0 | 34.13662 | 43 |
ATTAATC | 1155 | 0.0 | 34.0896 | 33 |
GACGTTC | 1170 | 0.0 | 33.844856 | 26 |
CGTTCAT | 1165 | 0.0 | 33.79699 | 28 |
CGAGACG | 1165 | 0.0 | 33.79699 | 23 |
TTCATTA | 1175 | 0.0 | 33.700836 | 30 |
TCTCGTA | 1165 | 0.0 | 33.603863 | 38 |
CATTAAT | 1175 | 0.0 | 33.509354 | 32 |
GTATGCC | 1175 | 0.0 | 33.509354 | 42 |
TCGTATG | 1175 | 0.0 | 33.509354 | 40 |
AATCTCG | 1175 | 0.0 | 33.31787 | 36 |
GTTCATT | 1190 | 0.0 | 33.086967 | 29 |
ATCTCGT | 1185 | 0.0 | 33.036705 | 37 |
TCATTAA | 1200 | 0.0 | 32.998734 | 31 |
ACGTTCA | 1195 | 0.0 | 32.948524 | 27 |