FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_10.35100000045076.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_10.35100000045076.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430185
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC67101.559794042098167RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT10070.23408533537896486No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG5270.1225054337087532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8000.036.84203745
CGTATGC8200.035.66907541
GCCCACG8400.035.62334418
CGAGACG8500.035.20424723
TATGCCG8400.035.08765443
ATGCCGT8450.034.88003544
AGACGTT8650.034.8538725
ATTAATC8700.034.6535633
GACGTTC8700.034.39495526
GTATGCC8600.034.27166442
TCGTATG8550.034.20893540
CCCACGA8750.034.1984119
CTCGTAT8700.034.13634539
CACGAGA8950.033.434221
AATCTCG8900.033.36923636
ACGTTCA9000.033.24845527
ACGAGAC9150.033.19517522
CGTTCAT9050.033.0647628
TCTCGTA9000.032.99846638
CCGAGCC9150.032.94928714
Produced by FastQC (version 0.11.2)