FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pc_1.35100000044f6d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pc_1.35100000044f6d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17529
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC537230.64635746477266No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT4772.721204860516858RNA PCR Primer, Index 44 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT1270.7245136630726224RNA PCR Primer, Index 44 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG1260.7188088310799247No Hit
ATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTTC1190.67887500713104RNA PCR Primer, Index 37 (95% over 23bp)
TATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTT350.1996691197444235RNA PCR Primer, Index 37 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT240.13691596782474755RNA PCR Primer, Index 44 (95% over 23bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6650.041.61654345
ATGCCGT7350.037.95918344
CCGAGCC7500.036.89999814
TCCGAGC7500.036.89999813
CTCCGAG7500.036.89999812
CCACGAG7600.036.71052620
CACGAGA7600.036.71052621
ACGAGAC7600.036.71052622
GACCCTA7600.036.71052626
AGACCCT7600.036.71052625
GAGACCC7600.036.71052624
ATCTCGT7550.036.6556337
TATGCCG7550.036.6556343
AGAATAT7550.036.6556332
TATCTCG7550.036.6556336
CGAGCCC7550.036.6556315
GAATATC7550.036.6556333
TCTCCGA7550.036.6556311
CTAGAAT7550.036.6556330
ATATCTC7550.036.6556335
Produced by FastQC (version 0.11.2)