FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_96.35100000044f44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_96.35100000044f44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34107
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC891226.129533526841996No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT6852.008385375436127RNA PCR Primer, Index 41 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTTC3901.1434602867446564RNA PCR Primer, Index 34 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT3170.9294279766616823RNA PCR Primer, Index 41 (95% over 23bp)
TATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTT1410.4134048728999912RNA PCR Primer, Index 34 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCG1260.3694256311021198No Hit
CTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT430.12607382648723134RNA PCR Primer, Index 41 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCG360.10555018031489136No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10300.041.9403845
CCGTCTT356.0732527E-638.5701744
ATGCCGT11200.038.5701744
CGTCTTC356.0732527E-638.5701745
ATCTCGT11450.037.5315337
GGCATCT11400.037.4987834
GCATCTC11400.037.4987835
TATGCCG11550.037.4013843
TCTCGTA11550.037.4013838
TCGTATG11550.037.4013840
CTCGTAT11550.037.4013839
CATCTCG11500.037.36834736
GTATGCC11600.037.24016642
CGTATGC11600.037.24016641
GAGACTC11600.037.24016624
ACGAGAC11550.037.2065822
CGAGCCC11550.037.2065815
AGACTCG11550.037.2065825
GACTCGA11550.037.2065826
AGCCCAC11500.037.17270317