Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_0113_pb_96.35100000044f44.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 34107 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 52 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC | 8912 | 26.129533526841996 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT | 685 | 2.008385375436127 | RNA PCR Primer, Index 41 (95% over 21bp) |
ATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTTC | 390 | 1.1434602867446564 | RNA PCR Primer, Index 34 (95% over 23bp) |
TTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT | 317 | 0.9294279766616823 | RNA PCR Primer, Index 41 (95% over 23bp) |
TATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTT | 141 | 0.4134048728999912 | RNA PCR Primer, Index 34 (95% over 22bp) |
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCG | 126 | 0.3694256311021198 | No Hit |
CTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT | 43 | 0.12607382648723134 | RNA PCR Primer, Index 41 (95% over 23bp) |
TCTTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCG | 36 | 0.10555018031489136 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 1030 | 0.0 | 41.94038 | 45 |
CCGTCTT | 35 | 6.0732527E-6 | 38.57017 | 44 |
ATGCCGT | 1120 | 0.0 | 38.57017 | 44 |
CGTCTTC | 35 | 6.0732527E-6 | 38.57017 | 45 |
ATCTCGT | 1145 | 0.0 | 37.53153 | 37 |
GGCATCT | 1140 | 0.0 | 37.49878 | 34 |
GCATCTC | 1140 | 0.0 | 37.49878 | 35 |
TATGCCG | 1155 | 0.0 | 37.40138 | 43 |
TCTCGTA | 1155 | 0.0 | 37.40138 | 38 |
TCGTATG | 1155 | 0.0 | 37.40138 | 40 |
CTCGTAT | 1155 | 0.0 | 37.40138 | 39 |
CATCTCG | 1150 | 0.0 | 37.368347 | 36 |
GTATGCC | 1160 | 0.0 | 37.240166 | 42 |
CGTATGC | 1160 | 0.0 | 37.240166 | 41 |
GAGACTC | 1160 | 0.0 | 37.240166 | 24 |
ACGAGAC | 1155 | 0.0 | 37.20658 | 22 |
CGAGCCC | 1155 | 0.0 | 37.20658 | 15 |
AGACTCG | 1155 | 0.0 | 37.20658 | 25 |
GACTCGA | 1155 | 0.0 | 37.20658 | 26 |
AGCCCAC | 1150 | 0.0 | 37.172703 | 17 |