FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_95.35100000044f2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_95.35100000044f2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11594
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC332528.67862687597033No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT2121.8285319993099878RNA PCR Primer, Index 41 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCG1851.5956529239261688No Hit
TTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT330.2846299810246679RNA PCR Primer, Index 41 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTTC170.1466275659824047RNA PCR Primer, Index 34 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4100.042.80488245
ATGCCGT4600.038.15217644
GTATGCC4800.036.56250442
TATGCCG4800.036.56250443
AGCCCAC4850.036.1855717
GCCCACG4850.036.1855718
CATCTCG4850.036.1855736
GAGCCCA4850.036.1855716
CCCACGA4850.036.1855719
ACGAGAC4850.036.1855722
CGTATGC4850.036.1855741
CGAGCCC4850.036.1855715
TCGTATG4850.036.1855740
CGAGACT4850.036.1855723
CTCGTAT4850.036.1855739
GAGACTC4850.036.1855724
CTCCGAG4850.036.1855712
ACTCGAC4800.036.09375427
AGACTCG4800.036.09375425
GACTCGA4800.036.09375426
Produced by FastQC (version 0.11.2)