FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_93.35100000044ed8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_93.35100000044ed8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28108
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC24638.762629856268678No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT3021.0744272093354206RNA PCR Primer, Index 41 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT820.2917318912765049RNA PCR Primer, Index 41 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTTC500.17788529955884447RNA PCR Primer, Index 34 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCG470.16721218158531378No Hit
TATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTT290.10317347374412977RNA PCR Primer, Index 34 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3100.039.1904545
CTCCGAG3350.036.2657912
TATGCCG3400.035.73246843
CATCTCG3400.035.73246836
TCTCGTA3400.035.73246838
TCGTATG3400.035.73246840
TCTCCGA3400.035.73246811
CGAGACT3400.035.73246823
AGACTCG3400.035.73246825
GACTCGA3400.035.73246826
CTCGTAT3400.035.73246839
CTCGACG3350.035.594228
GGCATCT3350.035.594234
TCGACGG3350.035.594229
CCACGAG3350.035.594220
GACGGCA3350.035.594231
CCCACGA3350.035.594219
CGAGCCC3350.035.594215
CCGAGCC3350.035.594214
CGGCATC3350.035.594233