Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_0113_pb_93.35100000044ed8.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 28108 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 38 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC | 2463 | 8.762629856268678 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT | 302 | 1.0744272093354206 | RNA PCR Primer, Index 41 (95% over 21bp) |
TTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT | 82 | 0.2917318912765049 | RNA PCR Primer, Index 41 (95% over 23bp) |
ATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTTC | 50 | 0.17788529955884447 | RNA PCR Primer, Index 34 (95% over 23bp) |
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCG | 47 | 0.16721218158531378 | No Hit |
TATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTT | 29 | 0.10317347374412977 | RNA PCR Primer, Index 34 (95% over 22bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 310 | 0.0 | 39.19045 | 45 |
CTCCGAG | 335 | 0.0 | 36.26579 | 12 |
TATGCCG | 340 | 0.0 | 35.732468 | 43 |
CATCTCG | 340 | 0.0 | 35.732468 | 36 |
TCTCGTA | 340 | 0.0 | 35.732468 | 38 |
TCGTATG | 340 | 0.0 | 35.732468 | 40 |
TCTCCGA | 340 | 0.0 | 35.732468 | 11 |
CGAGACT | 340 | 0.0 | 35.732468 | 23 |
AGACTCG | 340 | 0.0 | 35.732468 | 25 |
GACTCGA | 340 | 0.0 | 35.732468 | 26 |
CTCGTAT | 340 | 0.0 | 35.732468 | 39 |
CTCGACG | 335 | 0.0 | 35.5942 | 28 |
GGCATCT | 335 | 0.0 | 35.5942 | 34 |
TCGACGG | 335 | 0.0 | 35.5942 | 29 |
CCACGAG | 335 | 0.0 | 35.5942 | 20 |
GACGGCA | 335 | 0.0 | 35.5942 | 31 |
CCCACGA | 335 | 0.0 | 35.5942 | 19 |
CGAGCCC | 335 | 0.0 | 35.5942 | 15 |
CCGAGCC | 335 | 0.0 | 35.5942 | 14 |
CGGCATC | 335 | 0.0 | 35.5942 | 33 |