H23LNBCXX_l01n01_bir_0113_pb_92.35100000044ebe.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0113_pb_92.35100000044ebe.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	40
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATACGATCTCAT	1	2.5	Illumina PCR Primer Index 8 (96% over 27bp)
CTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGG	1	2.5	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATC	1	2.5	No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATAGAAAACT	1	2.5	RNA PCR Primer, Index 27 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTCTACGGCATCTCGTATGCCGTC	1	2.5	No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAAC	1	2.5	No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAACAAAAATCAGGTTCTCT	1	2.5	RNA PCR Primer, Index 27 (96% over 28bp)
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGTAAGAC	1	2.5	Illumina PCR Primer Index 8 (96% over 27bp)
TCTTATACACGAGCCCACGAGACCATCAGACATCTCGTATGCCGTCTTCTG	1	2.5	Illumina PCR Primer Index 3 (95% over 24bp)
AATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGATT	1	2.5	No Hit
GGGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGAT	1	2.5	No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACAAAAAC	1	2.5	Illumina PCR Primer Index 8 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC	1	2.5	No Hit
CTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTC	1	2.5	No Hit
CCACCGCAATTGATCCGAAAATCGCAACCACAGCAGCCATTACCACCACCT	1	2.5	No Hit
ATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTT	1	2.5	No Hit
TCGTTACTCATGTCAGCATTCTCACTTCTGATATCTCCAGGTCTTGTCACC	1	2.5	No Hit
ATATAAAATCAACTATAAAATGACTGAATCGAGCTCCCTTTTAATTTACGA	1	2.5	No Hit
CTTATACACATCTCCGAGCCCACGAGTATGATCTCGTATGCCGTCTTCTGC	1	2.5	RNA PCR Primer, Index 22 (96% over 26bp)
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATACTTATTCTT	1	2.5	RNA PCR Primer, Index 27 (96% over 28bp)
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACACT	1	2.5	No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATCACTAAGTTC	1	2.5	Illumina PCR Primer Index 5 (96% over 28bp)
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGAAAAAAA	1	2.5	Illumina PCR Primer Index 8 (96% over 27bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTATACCAGTGTTT	1	2.5	No Hit
GTTTCCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATACTGCTG	1	2.5	No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATAAAAACAA	1	2.5	Illumina PCR Primer Index 8 (96% over 27bp)
GCTCTTAAGTGTAGCAGAAAAGACAACTGGTCTCTATTTCTCCTGTCTCTT	1	2.5	No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATCTAAGACAC	1	2.5	RNA PCR Primer, Index 36 (96% over 29bp)
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACCCACCCGTCA	1	2.5	RNA PCR Primer, Index 27 (96% over 28bp)
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAGT	1	2.5	RNA PCR Primer, Index 27 (96% over 28bp)
GTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATA	1	2.5	No Hit
GGGCAAAGGGAATGCCATCGGTGCTGGGACCTGTGAGCACTACAGGAGGAA	1	2.5	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCATCGTTATCTCGTATGCCGTC	1	2.5	No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGTAGCTGGAAC	1	2.5	Illumina PCR Primer Index 5 (96% over 28bp)
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAACGCCGAG	1	2.5	RNA PCR Primer, Index 36 (96% over 29bp)
AACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGA	1	2.5	No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACACCTA	1	2.5	RNA PCR Primer, Index 27 (96% over 28bp)
GTTTTTAATCCATCTTGAGTTGGTTTTTGTACATAATGAACTGTCTCTTAT	1	2.5	No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGCATTTGGGTT	1	2.5	Illumina PCR Primer Index 8 (96% over 27bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCACCCGTACTCTGC	1	2.5	No Hit

Adapter Content

Adapter graph

Kmer Content

No overrepresented Kmers

Summary