FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_91.35100000044e95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_91.35100000044e95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC57.042253521126761No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCA22.8169014084507045No Hit
ACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAC11.4084507042253522No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACAGCCCCC11.4084507042253522No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCAT11.4084507042253522No Hit
AACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATCGGTCAATGA11.4084507042253522No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAG11.4084507042253522No Hit
CCTTTGTTCCTTCGGACCTCTCGCCCAAATGAAATGGGATGAATCCAATCA11.4084507042253522No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATCTATAAAA11.4084507042253522Illumina PCR Primer Index 8 (96% over 27bp)
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC11.4084507042253522No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACACACCTTGA11.4084507042253522Illumina PCR Primer Index 8 (96% over 27bp)
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGTCTCTTATA11.4084507042253522No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGGACACT11.4084507042253522RNA PCR Primer, Index 27 (96% over 28bp)
GACGTGGGCATCGACGGCGCCGGCGCGTCCGCCGACGAGGCGGCCATGCAC11.4084507042253522No Hit
TTTTTAGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATAC11.4084507042253522No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCTTTAAT11.4084507042253522No Hit
CTTATACACATCTCCGAGCCCACGAGACTGATTAGAATCTCGTATGCCGTC11.4084507042253522No Hit
GTATCAACGCAGAGTACGGGTTCATGAAGGCGATGCGTTGTCATGGATGAA11.4084507042253522No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTATAC11.4084507042253522No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCTGTCTCTTATACACGTCTCCGAG11.4084507042253522No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTCTTTCTCTTATT11.4084507042253522No Hit
GCTGGGACATGATTTTTAACTAGCCACAGGCATAAAGGGTTAGGAAACAAG11.4084507042253522No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACTATGCCAAA11.4084507042253522Illumina PCR Primer Index 8 (96% over 27bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTGCGTTTATACCACCC11.4084507042253522No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTATTTGACCCATATTCCGCG11.4084507042253522No Hit
CGGCTGGCCGAGGCCCGCTCGCTGCTGCGCTCGGCCGACGCTGCGCGCTCG11.4084507042253522No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT11.4084507042253522No Hit
AGTTCACGTGGAGCCGTCCTTGAAATACCACCCTGGTGTGTTCTGTCTCTT11.4084507042253522No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAGAGA11.4084507042253522RNA PCR Primer, Index 27 (96% over 28bp)
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGACATTAGCTC11.4084507042253522Illumina PCR Primer Index 8 (96% over 27bp)
ATATCTCTCTATTATGTTCTGCTTTTTTCTTAACAGGCTGCAGCATACTAC11.4084507042253522No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGAAAGAAACCAA11.4084507042253522RNA PCR Primer, Index 36 (96% over 29bp)
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAAAAGAA11.4084507042253522RNA PCR Primer, Index 27 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTTT11.4084507042253522RNA PCR Primer, Index 34 (95% over 22bp)
ATACACATCTCCGAGCCCAGGAGACTCGACGGCATCTCGTATGCCGTCTTC11.4084507042253522RNA PCR Primer, Index 41 (96% over 25bp)
CATCGTTGCTGATTGCCGGTGACTGGCTGGGGGTGGCCGGGCTTGCGCTTG11.4084507042253522No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCATAGCATCTCGTATGCCGTC11.4084507042253522No Hit
GTGCAACGGTACGCCGAGGCTATCGACGGCGCGCTCCCAGCGGGCTTGCCA11.4084507042253522No Hit
GGTTTCTCAATCTCAGCAGGCACAACACGCTTGGTAGATATGGGATATTGC11.4084507042253522No Hit
CTTATACACATCCCCGAGCCCACGAGACTCCACAGCATCTCGTATGCCGTC11.4084507042253522RNA PCR Primer, Index 43 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTTC11.4084507042253522RNA PCR Primer, Index 34 (95% over 23bp)
TCTCGATTGCTTCAACATAAGCGTCCTCATCGAGAACTCTCATCCGTTTTC11.4084507042253522No Hit
CCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCTGTCTCTTAT11.4084507042253522No Hit
AAAACGGTCTTCACTCCCAAGCTCAAGTGGTCACAGATCACCTTGATGCAC11.4084507042253522No Hit
GGTATCAACGCAGAGTACGGGGAAGAAGCACCGGCTAACTCCGTGCCAGCA11.4084507042253522No Hit
ACAGAGAGGTTTAGATCAAGAGGCAGAGTCCTGTCTCTTATACACATCTCC11.4084507042253522No Hit
GGCCTTCCCACATCGTTTCCCACTTAACCATGACTTTGGGACCTTAGCTGG11.4084507042253522No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTGCCGACCTGCACGAATG11.4084507042253522No Hit
GGGCGTGGACCGACGCGGAATACGGTGGCGGCCTAAGCCCGGGCTTTTGAT11.4084507042253522No Hit
TGCTACTACCACCAAGATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGC11.4084507042253522No Hit
CCATAGGCACGCACGCCGCTCCATTTTCCCGTCATGCCGAACGCGGGCATG11.4084507042253522No Hit
CTCAACAACCATGGGCTTGGTTGGGGTCATCTTAACCATACCAGCGTAACC11.4084507042253522No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTTAGTATCTCGTATGCCGTC11.4084507042253522No Hit
GGGTTGGGTAACCTCCTGAAACTCCGTCGTGATGCTGTCTCTTATACACAT11.4084507042253522No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATCCC11.4084507042253522No Hit
GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCAACG11.4084507042253522No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC11.4084507042253522No Hit
ACGGGAAGCAGTGGTATCAACGCAGAGTACCCGTCTCTTATACACATCTCC11.4084507042253522No Hit
GAAAAAAAGTACTCTGCGTCCCGTACTCTGCGTTGATACCTGTCTCTTATA11.4084507042253522No Hit
ACTATAAGGAACCAACGATTCTCTCTTCTTAAACAACCTATATCCTCCACA11.4084507042253522No Hit
AACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGC11.4084507042253522No Hit
AAAAAGTACTCTGCGTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACA11.4084507042253522No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACG11.4084507042253522No Hit
AACCGGGACGTGGCGGTTGACGGCAACGTTAGGGAGTCCGGAGACAGTCCC11.4084507042253522No Hit
TTTTTTTTTCACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTCAA11.4084507042253522No Hit
ATATTAATGTTGATCACATTTAAAGTCTAGACATTGAAGCTGTCCCTTATA11.4084507042253522No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCTC50.045.03
CAGCATA50.045.041
TTTCTTA50.045.026
TATGTTC50.045.013
GTTCTGC50.045.016
CTCTCTA50.045.05
TTTTCTT50.045.025
TGTTCTG50.045.015
TTTTTTC50.045.023
TTTTTCT50.045.024
TTCTGCT50.045.017
GGCTGCA50.045.036
TCTATTA50.045.08
CTCTATT50.045.07
ATTATGT50.045.011
TCTGCTT50.045.018
GCAGCAT50.045.040
TGCTTTT50.045.020
GCTGCAG50.045.037
TATCTCT50.045.02
Produced by FastQC (version 0.11.2)