FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_90.35100000044e88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_90.35100000044e88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC22.9850746268656714No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCATCATCTCGTATGCCGTC22.9850746268656714No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAA11.4925373134328357No Hit
GTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACAAACCGCCTGC11.4925373134328357No Hit
GAACCTGCGTCAGCAATTTTTTCATCGTAAGCAAGGATTTCAAGGAAGAAT11.4925373134328357No Hit
CTTATACACATCTCCGAGCACACGAGACTCGACGGCATCTCGTATGCCGTC11.4925373134328357RNA PCR Primer, Index 41 (95% over 22bp)
CGGGACGTAGAGTACGGGACGCAGAGTACGGGGAGCTGTCTCTTATACACA11.4925373134328357No Hit
CCAGAGGATGATGTTTGCCCGATCTGTTTCGGCTCCTTTACGGTTCCGTGT11.4925373134328357No Hit
CTGCCTGTACGTTCCTCTTCCACAGCATTTTGTTGTATGCGGGAGAGTAGC11.4925373134328357No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA11.4925373134328357No Hit
CCACCACCCCCAAGTACTCCAAGGCCAGGTACGATGAAATCATCAAGGAGG11.4925373134328357No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACCTTCCC11.4925373134328357No Hit
CTCTTATACACACCTCAGAGCCCACGAGACTCAACTGTATCTCGTATGCCG11.4925373134328357No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.4925373134328357No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAATCAGCCAC11.4925373134328357Illumina PCR Primer Index 8 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC11.4925373134328357No Hit
ACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTA11.4925373134328357No Hit
GGGACCTTAGCGTACGATCTGGACTGTTTCCCTCTCGACTTTGGATCTTAG11.4925373134328357No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACT11.4925373134328357No Hit
ATGTCACACCTGCCGATGAAACTCCTGCGTAAGAAGATCAAGAAGCGGAAC11.4925373134328357No Hit
CTCCTAGGAGGGCTTGCTGTGCGGAGGGCCTGCTATGGTGTGCTGCGGTTC11.4925373134328357No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGTTTATAACCCGCCTTC11.4925373134328357No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCTGGCATCTCGTATGCCGTC11.4925373134328357No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATTTAGTAAC11.4925373134328357RNA PCR Primer, Index 27 (96% over 28bp)
ACCAGAGGCTGTTCACCTTGGAGACCTGATACGGTTATGAGTACGACCGGG11.4925373134328357No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGTTATTTCTCCCGTT11.4925373134328357No Hit
GGTCATCGTCGAATCTCCGTTCGTCATATCAAAGCTTATCTTCAATCGTCG11.4925373134328357No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCCTGTCTCTTAT11.4925373134328357No Hit
GTCCTGGTCGGATGCGGAACGGAGCAATCCGGTCCGCCGATCGATTCGGGG11.4925373134328357No Hit
AAAAGTACTCTGCGTTGATACCTGTCTATTATACACATCCTCCGAGACCAC11.4925373134328357No Hit
ACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTAGCAACGC11.4925373134328357No Hit
GTAATTTAGAAGCTTTTGATTCTGGAACTGTAAAAATAGATGGCAAACAAA11.4925373134328357No Hit
GTACGGGACGGGTGGGAGAAACGCAATGATGTGGTCGTCTTATCGAACGTT11.4925373134328357No Hit
CTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTA11.4925373134328357No Hit
CACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCACTCGCCGCTACTGG11.4925373134328357No Hit
ACAGACAATGGCGGCGGCGGCGGCGAAAGCAGCGGTGGTTTTGCCACGGCC11.4925373134328357No Hit
CGTTACTTATGTCAGCATTCGCACTTCTGACTGTCTCTTATACACATCTCC11.4925373134328357No Hit
GATGGGGGCCGGCGATGCGTCCTGGTCGGATGCGGAACGGAGCAATCCGGT11.4925373134328357No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACTGTATCTCGTATGCCGTC11.4925373134328357No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACTTACGCCATC11.4925373134328357RNA PCR Primer, Index 27 (96% over 28bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTAATACCCCCCG11.4925373134328357No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGCCGTC11.4925373134328357No Hit
AGCATATTCTGTTTCAACAAGACCAAGTTCTGAACAATTACGCAGCTAGTA11.4925373134328357No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGT11.4925373134328357No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTACGTCATCTCGTATGCCGTC11.4925373134328357No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGCGACAA11.4925373134328357RNA PCR Primer, Index 36 (96% over 29bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTTTCCCCCCGGG11.4925373134328357No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTCATCTCCGA11.4925373134328357No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGATACAACG11.4925373134328357RNA PCR Primer, Index 36 (96% over 29bp)
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAACTGCA11.4925373134328357Illumina PCR Primer Index 8 (96% over 27bp)
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT11.4925373134328357No Hit
ACCCTATTCTTATTCTCTCTCTGTATTTTCCACAGTGCTTCCTCTTCCAGC11.4925373134328357No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAACACG11.4925373134328357RNA PCR Primer, Index 27 (96% over 28bp)
GGTTAAGGGAAAGCGCCGAGATGACGGGCTTTCTGCTGCCGCCCGCAAGCA11.4925373134328357No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAATACTTTCC11.4925373134328357No Hit
TTGGAACTTTGCTTTTAGCAGGAAAGGTTGAATGCAGGAATGTGTGCACAA11.4925373134328357No Hit
CTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAA11.4925373134328357No Hit
CTTATACACATCTCAGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC11.4925373134328357No Hit
TGCGTGGACTGCTCGGTCACGATCCTCTTGGTGACGGGTTATTCCAGCCTA11.4925373134328357No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC11.4925373134328357No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGATGGCATCTCGTATGCCGTC11.4925373134328357No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCTTTCCCCTCC11.4925373134328357No Hit
GGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA11.4925373134328357No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATTAGCATCCCG11.4925373134328357Illumina PCR Primer Index 8 (96% over 27bp)
CTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC11.4925373134328357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCGG50.045.013
ACAAACC50.045.039
AAACCGC50.045.041
GTTGAGC50.045.09
TTCACAT50.045.024
TCCCAGG50.045.03
GGTTGAG50.045.08
AACAAAC50.045.038
AACCGCC50.045.042
CCCAGGT50.045.04
CACATCT50.045.026
TGACTTA50.045.032
CGCCTGC50.045.045
CCCGGGG50.045.015
CGGGGAT50.045.017
CCGCCTG50.045.044
GTTCCCA50.045.01
CATCTGA50.045.028
GGATTTC50.045.020
ACCGCCT50.045.043