H23LNBCXX_l01n01_bir_0113_pb_9.3510000004445c.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0113_pb_9.3510000004445c.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25644
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC	7311	28.509592887225082	RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT	790	3.0806426454531275	No Hit
ATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCTTC	200	0.7799095304944627	RNA PCR Primer, Index 45 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG	172	0.6707221962252379	No Hit
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT	149	0.5810326002183747	No Hit
TATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCTT	58	0.2261737638433942	RNA PCR Primer, Index 45 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55	0.21447512088597723	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	53	0.2066760255810326	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	53	0.2066760255810326	No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	47	0.1832787396661987	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	47	0.1832787396661987	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	45	0.1754796443612541	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34	0.13258462018405864	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	33	0.12868507253158634	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32	0.12478552487911403	No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA	29	0.1130868819216971	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	28	0.10918733426922478	No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26	0.10138823896428013	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCTT	20	6.904691E-4	45.0	44
CGTCTTC	20	6.904691E-4	45.0	45
TGCCGTC	810	0.0	41.666668	45
ATGCCGT	885	0.0	38.135593	44
CCCACGA	900	0.0	37.5	19
AATCTCG	895	0.0	37.4581	36
ATCTCGT	895	0.0	37.4581	37
TCTCGTA	895	0.0	37.4581	38
TCGTATG	895	0.0	37.4581	40
CTCGTAT	895	0.0	37.4581	39
GTATGCC	905	0.0	37.29282	42
CCACGAG	905	0.0	37.29282	20
TATGCCG	905	0.0	37.29282	43
CACGAGA	905	0.0	37.29282	21
CGTTCAT	900	0.0	37.25	28
GCCCACG	900	0.0	37.25	18
ACGTTCA	900	0.0	37.25	27
GTTCATT	900	0.0	37.25	29
CATTAAT	900	0.0	37.25	32
GACGTTC	900	0.0	37.25	26

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content