FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_89.35100000044e6e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_89.35100000044e6e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92
Sequences flagged as poor quality0
Sequence length51
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC55.434782608695652No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA22.1739130434782608No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA22.1739130434782608No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA22.1739130434782608No Hit
GTGGTATCAACGCAGAGTACGGGGCTGTCTCTTATACACATCTCCGAGCCC22.1739130434782608No Hit
GTGGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAA22.1739130434782608No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA11.0869565217391304No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAAGAACTTAC11.0869565217391304RNA PCR Primer, Index 27 (96% over 28bp)
AGAGGAAAGAAACAAATTGCAGAGAAATAGCTGTCTCTTATACACATCTCC11.0869565217391304No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.0869565217391304No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAA11.0869565217391304No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCTTTTACCCCCCCCCCGC11.0869565217391304No Hit
CCCAGTGTGTTTCGACACACTATCATTAACTTAATCCATAGGTTAATGAGG11.0869565217391304No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTCCGTTGATACCCCCCTTACTCC11.0869565217391304No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTT11.0869565217391304No Hit
TATCAAAGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGT11.0869565217391304No Hit
AGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.0869565217391304No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTAAACCCCCCGTTCC11.0869565217391304No Hit
GAGTACGGGGTGGTATCAACGCAGAAAACTGTCTCTTATACACATCTCCGA11.0869565217391304No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA11.0869565217391304No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAACTGCATCTCGTATGCCGTC11.0869565217391304No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTT11.0869565217391304No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTAATACCCCCCCC11.0869565217391304No Hit
CTCCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCAC11.0869565217391304No Hit
GAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA11.0869565217391304No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT11.0869565217391304No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTAAACC11.0869565217391304No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTT11.0869565217391304No Hit
GTATCAACGCAGAGTACGGACTGTCTCTTATACACATCTCGGAGCCCACGA11.0869565217391304No Hit
ACCCCGTACTCTGCGTTGATACCACTGTCTCTTATACACATCTCCGAGCCC11.0869565217391304No Hit
GTACTTTTTTTTTTTTTTTTTTTTTGATACCCCTCCTTCCCGTACCTGTCT11.0869565217391304No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAACAAAAAAAAAAAA11.0869565217391304No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTATACCCC11.0869565217391304No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.0869565217391304No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTATCC11.0869565217391304No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGTGTGGTATCAACGC11.0869565217391304No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATCAAAACC11.0869565217391304Illumina PCR Primer Index 8 (96% over 27bp)
TATCAACGCAGAGTACGGGGTGGTATCAACGAAAAAAAAAAAAAAAAAAAA11.0869565217391304No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTT11.0869565217391304No Hit
CTTATACACATCTCCGAGCCCACGAGCGAGAGTTGATCTCGTATGCCGTCT11.0869565217391304No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG11.0869565217391304No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACGAACATT11.0869565217391304Illumina PCR Primer Index 8 (96% over 27bp)
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAGTA11.0869565217391304No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA11.0869565217391304No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA11.0869565217391304No Hit
ACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.0869565217391304No Hit
ATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGAGC11.0869565217391304No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGCTTTGAAACCCCCCCGTAC11.0869565217391304No Hit
GTATCAACGCAGAGTACGGGGGCTGCAACTGTTTATTAAAAACACAGCACT11.0869565217391304No Hit
GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC11.0869565217391304No Hit
ATATAAATCTCGTATGCCGCCTTCTGCTTGAAAAAAAAAACTTCATCCGTC11.0869565217391304Illumina PCR Primer Index 4 (96% over 25bp)
CCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA11.0869565217391304No Hit
CTTATCACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT11.0869565217391304RNA PCR Primer, Index 41 (95% over 23bp)
GTGCTTGGCGCCTGCGCCGGCTGGCGGTGCGGCGCTGCCGCCTCAGCCATG11.0869565217391304No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAACAC11.0869565217391304Illumina PCR Primer Index 8 (96% over 27bp)
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACA11.0869565217391304No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTATACCCCCCGTAC11.0869565217391304No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTC11.0869565217391304No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATACCCCTGC11.0869565217391304No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTTCCCCCCC11.0869565217391304No Hit
GTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA11.0869565217391304No Hit
CTTATACACATCTCCGAGCCCATGAGACGTCTTAGTATCTCGTATGCCGTC11.0869565217391304No Hit
GTAATACACACCGTCCTCTGCGTAACCCCCGTACTCTGCGTTGATACCCTG11.0869565217391304No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCCCGTACCTTTCT11.0869565217391304No Hit
CCACCCGTACTCTGCGTTTGATACCCTGTCTCTTATACACATCTCCGAGCC11.0869565217391304No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTAACCCCCCCCC11.0869565217391304No Hit
GTACTTTTTTTTTTTTTTTTTTTTCGGTTATACCTTTTTCTTATACACAAT11.0869565217391304No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATCAAACACA11.0869565217391304RNA PCR Primer, Index 27 (96% over 28bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT11.0869565217391304No Hit
TCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCCTGTTTCTTATACACATC11.0869565217391304No Hit
GAGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT11.0869565217391304No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTCGTTTATACCC11.0869565217391304No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAACTC11.0869565217391304No Hit
GCAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTCC11.0869565217391304No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTATACCCCTCCCC11.0869565217391304No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.0869565217391304No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTATACC11.0869565217391304No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTATTGACTGTCCCC11.0869565217391304No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAGTA11.0869565217391304No Hit
ATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA11.0869565217391304No Hit
TTTTTCTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTA11.0869565217391304No Hit
ATTTAGAGAAATTCCTGAGAAAGTAATTCGTAGAATTGATGCTTCTTGTAA11.0869565217391304No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAACCATCGTTT11.0869565217391304No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAA50.045.019
GGGCAGT50.045.06
TGGTATC50.045.012
ACGCAAA50.045.020
TACGGGC50.045.03
AGTGGTA50.045.010
GTATCAA50.045.014
TCAACGC50.045.017
AGTACGG50.045.01
CGGGCAG50.045.05
GCAGTGG50.045.08
GCAAAAA50.045.022
ACGGGCA50.045.04
TATCAAC50.045.015
GTGGTAT50.045.011
ATCAACG50.045.016
GGCAGTG50.045.07
CAGTGGT50.045.09
GGTATCA50.045.013
CGCAAAA50.045.021
Produced by FastQC (version 0.11.2)