FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_88.35100000044e45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_88.35100000044e45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159281
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC3172819.91951331295007No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT49753.123410827405654No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCG9550.5995693146075175No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCT6700.4206402521330228No Hit
ATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCTTC3450.21659833878491472Illumina PCR Primer Index 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA207.015217E-444.99968724
TGATACG207.015217E-444.99968719
GACACGA207.015217E-444.9996878
GAATCCC207.015217E-444.99968736
TGCCGTC34800.042.8660845
ATGCCGT40200.037.10795244
GAGCCCA41450.036.04317516
AGCCCAC41400.036.03235617
GATACGG250.002101746435.99974420
GGAACGA250.002101746435.99974440
AGACACG250.002101746435.9997447
GAACGAA250.002101746435.99974441
ATACGGA250.002101746435.99974421
TCCGAGC41650.035.92411813
CTCCGAG41600.035.9132112
CGAGCCC41600.035.9132115
GCCCACG41550.035.9022818
CACGAGA41500.035.89131521
CCCACGA41450.035.8803319
CCGAGCC41650.035.870114
Produced by FastQC (version 0.11.2)