FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_87.35100000044e2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_87.35100000044e2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181566
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC4164622.93711377680843No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT118396.520493925074078No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCG12570.6923102342949671No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCT10280.5661852990097265No Hit
ATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCTTC3340.18395514578720687Illumina PCR Primer Index 1 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCG2090.11510965709438993No Hit
TATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCTT1920.10574667063216682Illumina PCR Primer Index 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC45800.042.2486545
TCCCCTG250.002102512235.9997837
CTTTACG250.002102512235.999789
CGTCTTC458.6468026E-734.99978645
TATCTCG401.5534324E-533.74979436
ATGCCGT58750.032.8976744
GCCCACG60950.032.00554718
AGCCCAC60950.031.96863217
GAGCCCA60950.031.96863216
CCGAGCC61200.031.94833414
CCCACGA60950.031.93171719
CGAGCCC60950.031.93171715
CCACGAG60950.031.93171720
CACGAGA60900.031.92098821
GCCGTCT2750.031.90889545
ACGAGAC61000.031.90554222
CGAGACT60900.031.88404323
TCCGAGC61200.031.87480513
GACTAAG60800.031.82546226
GATCTCG60000.031.79980536
Produced by FastQC (version 0.11.2)