FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_86.35100000044e01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_86.35100000044e01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131494
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC2527419.22064885089814No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT56964.331756582049371No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCT7310.5559189012426423No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCG6690.5087684609183689No Hit
ATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCTTC2710.2060930536754529Illumina PCR Primer Index 1 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCTT1550.11787610081068338Illumina PCR Primer Index 1 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCG1490.11331315497285048No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAA207.0122356E-444.9977227
CCGTCTA207.0122356E-444.9977226
TGCCGTC28500.041.60315345
TTCATGC250.002096942236.011876
ACACCGT250.00210086435.99817723
CGTCTTC250.00210086435.99817745
TCATGCA250.00210086435.9981777
ACCGTCT250.00210086435.99817725
GCCGTCT2000.035.99817745
AACACCG250.00210086435.99817722
ATGCCGT35100.033.6521444
GCCCACG36150.033.04811518
CCGAGCC36250.032.95694714
CCCACGA36200.032.9403219
ACGAGAC36200.032.9403222
TCCGAGC36150.032.9236413
CCACGAG36150.032.9236420
CACGAGA36250.032.89488221
AGCCCAC36400.032.88294617
CGAGCCC36400.032.88294615
Produced by FastQC (version 0.11.2)