FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_85.35100000044de6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_85.35100000044de6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197042
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC8323942.24429309487318No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT191989.7431004557404No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCG32061.6270642807117264No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCT8810.44711279828665973No Hit
ATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCTTC3080.1563118522954497Illumina PCR Primer Index 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCCC253.880169E-544.99771515
TGCCGTC89750.043.71922745
CAGAGCC301.1368311E-437.49809614
ATGCCGT110400.035.52129444
TATGCCG113650.034.5055143
CTCGTAT113300.034.49295439
ATCTCGT113100.034.47437737
TCTCGTA113100.034.47437738
GATCTCG112900.034.45573436
GTATGCC113850.034.44489342
GAGACTA114100.034.42857724
TGATCTC113000.034.42524335
GAGCCCA113800.034.42048616
ACGAGAC113800.034.42048622
CGAGACT113900.034.41001523
TCCGAGC113850.034.4053713
CCGAGCC113850.034.4053714
CGTATGC113800.034.40071541
CATGATC113350.034.39834233
CGAGCCC113750.034.39605315
Produced by FastQC (version 0.11.2)