FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_84.35100000044dcc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_84.35100000044dcc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC63.7267080745341614No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT31.8633540372670807No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACTCTTATACAC10.6211180124223602Illumina PCR Primer Index 8 (96% over 27bp)
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGCCGTC10.6211180124223602RNA PCR Primer, Index 27 (96% over 28bp)
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACACAGAGTACGGGAA10.6211180124223602No Hit
GTTAAGTAACTTTCCCAAGAACACACAGTTATGATATTGGAACACCTGTCT10.6211180124223602No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATACTGTCTC10.6211180124223602Illumina PCR Primer Index 5 (96% over 28bp)
GTACTTTTTTTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCCC10.6211180124223602No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACTTTTAGATGA10.6211180124223602Illumina PCR Primer Index 5 (96% over 28bp)
GAGTACGGGCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAA10.6211180124223602No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAAAAAAA10.6211180124223602RNA PCR Primer, Index 27 (96% over 28bp)
ATCTTATGTGACATTGCATGAAGTTATGCTATGTGATCAGTAGCTATGCTG10.6211180124223602No Hit
GAAAAGAATAGAGAAGATAGGGAAATTAGAAGATAAAAACATACCTGTCTC10.6211180124223602No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCT10.6211180124223602No Hit
GTCTGATAGCGCTTAAGCGCGAACTTCGAAAGGGGATCCGGGTAAAATTCC10.6211180124223602No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAACAAACA10.6211180124223602Illumina PCR Primer Index 5 (96% over 28bp)
GACTGTTACATTTAGCAATCAACAGCATGGGGTGCAAAAAAAAAAAAAATC10.6211180124223602No Hit
ACATTACTCACCCGTCCGCCACTCGTCAGCAAAGAAGCAAGCTTCTTCCTG10.6211180124223602No Hit
CTCCACAGCTGTGTGAGCTGCCATGCTTCCAACCCTTCAAACCCAAGGCCG10.6211180124223602No Hit
CCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAG10.6211180124223602No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAACAAG10.6211180124223602RNA PCR Primer, Index 36 (96% over 29bp)
CTGTTAAACAGAAAAGATAACTCTTCCCGAGGCCCCCGCCGACGTCTCCGG10.6211180124223602No Hit
GTTTACGATAGGGATCTCAAAATGCTTCTGATCTGTCTCTTATACACATCT10.6211180124223602No Hit
GAATGTATTAAGCAGGTCAAGTTTAGGTTACTTCAACACTTCTTCTGGATG10.6211180124223602No Hit
ACCCCTACTTTCCTGGCCAGGCCATTGCCATGGCCCCTCCCATCTACACAG10.6211180124223602No Hit
TCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTATCCTGAGG10.6211180124223602No Hit
AAGCAATGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCC10.6211180124223602No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGAAGAAGTCG10.6211180124223602RNA PCR Primer, Index 27 (96% over 28bp)
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATATACAGATAA10.6211180124223602Illumina PCR Primer Index 8 (96% over 27bp)
ATGCAGATGGAATAGTAGAAGGCCTGGATAATGAACTTATTCCATCTCAGC10.6211180124223602No Hit
GGGGAAACCCAGTGTGTTTCGACACACTATCATTAACCTGTCTCTTATACA10.6211180124223602No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC10.6211180124223602No Hit
GCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGAC10.6211180124223602No Hit
ATTCAAAGGACACTGGGATATGAACTATCCCGTACTCCTGTCTCTTATACA10.6211180124223602No Hit
ACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCA10.6211180124223602No Hit
CCTCTAAGCATCAGGTAACATCAAATCGTACCCCAAACCGACACTGTCTCT10.6211180124223602No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAACTGTCTCTTA10.6211180124223602No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC10.6211180124223602No Hit
AGAAAGTATTCCTCAAGAAAGGCGGCCCTGGGGGGGACCCTACCGCATAAG10.6211180124223602No Hit
CGCTGATGCCGAGGCACGCCGTTAGGCGCGTGCTGCAGACCACAATCACGG10.6211180124223602No Hit
GAGTACGGGAAGCAGTGGTATCAAGCAGTGCTGTCTCTTATACACATCTCC10.6211180124223602No Hit
CAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACAAA10.6211180124223602No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATCACATATACA10.6211180124223602Illumina PCR Primer Index 8 (96% over 27bp)
GACACACTCATAAAAACTCCATATATCATCCTCAGAATAATCAGTTTCTGT10.6211180124223602No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGTAAGAAGG10.6211180124223602Illumina PCR Primer Index 5 (96% over 28bp)
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGATTGCTATG10.6211180124223602RNA PCR Primer, Index 27 (96% over 28bp)
AGAACCAGTAAGAGATTCTACATTGTATTAGTCCGTTTTCATGCTGCTGAT10.6211180124223602No Hit
GTGTGTTTCGACACACTATCATTAACCGCATCCACCGGCCGTCCCGTAAAC10.6211180124223602No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT10.6211180124223602No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGCGTCATCTCGTATGCCGTC10.6211180124223602No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGAAAGAAA10.6211180124223602Illumina PCR Primer Index 5 (96% over 28bp)
ATATTGGGTGTCATATTGAATGAAAACGTTTGTCTAAAACAAGATCTTTAA10.6211180124223602No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACACAAACAC10.6211180124223602RNA PCR Primer, Index 27 (96% over 28bp)
GAACAAAGATGTATCATCAGAAGTTTCTCCAAAGTTAAAGCGACATCAGAA10.6211180124223602No Hit
TTTAGAACATATTACCCTGTGACTTACGTAGGAAACCCTGTCTCTTATACA10.6211180124223602No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCATTATCTCGTATGCCGTC10.6211180124223602No Hit
CTTATACACATCTGCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC10.6211180124223602No Hit
GTTATAGTATGTTCACATAATGAATACTATACAGCCTGTCTCTTATACACA10.6211180124223602No Hit
ATCTTCCTCTTGACTTGGATTAGTGGTGCTGCCGTAACCCTGTCTCTTATA10.6211180124223602No Hit
TCCTTCCCCTTCTTCTTCTCCTTCTTCTTCTTCTTCTCCGTCTTCTTCTCC10.6211180124223602No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATAGCAAATA10.6211180124223602RNA PCR Primer, Index 27 (96% over 28bp)
GAGCTCAACTTCTCCTTGACCATCTTTGAGAGCTCAATGTAGCATTGCTGA10.6211180124223602No Hit
GAAAGCATGTTGTATGCTTACTAGGATCAAAGAATAAACAAGTACAAAACT10.6211180124223602No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTATGATCTCGTATGCCGTC10.6211180124223602No Hit
GTCTGAAATCATAGTCTGATACCAAGCCTTGCCTGCTGCTTTGATCGCTTG10.6211180124223602No Hit
CCCCATCAATTATCACCCTTCGATGCTACAGTAGACACCAAACTGTTTATG10.6211180124223602No Hit
GTATAGCGAAATGGTAAGAAATAAGGAATGTTATTAAAGATCCTTTGATAG10.6211180124223602No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT10.6211180124223602No Hit
GTACGGGAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAGTACC10.6211180124223602No Hit
ATGTATAAGTCTTGATTCAAAATCACAAGCATAGAGTTTGCAAAACTTAAA10.6211180124223602No Hit
GTATAGCAGCATGAGCTTTCTTATACATCTCCTCCATCATGTCTGGAGTTA10.6211180124223602No Hit
TATCAACGCAGAGTACGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTAT10.6211180124223602No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACCACACTC10.6211180124223602RNA PCR Primer, Index 27 (96% over 28bp)
AGTTAAGCGTGCATAAGTTAACTTCCAATTTACATACTCTGCTTAGAATCT10.6211180124223602No Hit
GTATTTATATCAGCGTGTAACGCAGTTATCTTTGAATAAATAGTTATGCCA10.6211180124223602No Hit
CTTATACACATCTCAGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC10.6211180124223602No Hit
GTTCTAGATAAATCCCTCCCTTTATTCATCCCATGTCATTACTGTCTCTTA10.6211180124223602No Hit
GAATACGGGAATGCCACGATGAATACGTTCCCGGGCCTTGTACCTGTCTCT10.6211180124223602No Hit
GATTATGAGGGCGTGATCATGAAAGGTGATAAGCTCTTCTATGATAGGGGA10.6211180124223602No Hit
TTTTTTTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAG10.6211180124223602No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTACC10.6211180124223602No Hit
CAATCTGATCGTGCCCTATTTCGATCGCGTCGGACGCAAGATCAACATGAT10.6211180124223602No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATCAAATACATAC10.6211180124223602RNA PCR Primer, Index 36 (96% over 29bp)
ACAATATCTTGATATTCAAAAGGAAGTATCTCTTACAGTAACAATTGGAAA10.6211180124223602No Hit
ACATAAAGCCAAGACATCGTTTCATGTCATCTTATGAGCAGAAAGTTCAAC10.6211180124223602No Hit
GTATAGAATCTCATAAGCATTAAGACTAAAACCTGTCTCTTATACACATCT10.6211180124223602No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGC10.6211180124223602No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGTACTGGGTA10.6211180124223602Illumina PCR Primer Index 8 (96% over 27bp)
GGGAAGAGCCCAGCGCCGAATCCCCGCCCCGCGGCGGGGCGCGGGACATGT10.6211180124223602No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAACTAGATC10.6211180124223602RNA PCR Primer, Index 27 (96% over 28bp)
CATGCAAGGTTTATTTACAAATCAAAAGCACAATGGAACAATACTCACAAC10.6211180124223602No Hit
GTTTTACACAGTACAGTAAGCACAATTGGAACCTGTCTCTTATACACATCT10.6211180124223602No Hit
GTTAAAGACTAATGCTCAGATGCTGTTTACGGATATTTATATTCCTGTCTC10.6211180124223602No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA10.6211180124223602No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGTATTAATGGA10.6211180124223602Illumina PCR Primer Index 5 (96% over 28bp)
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG10.6211180124223602No Hit
CTTATACACATCTCCGAGCCCACGAGCTGTTATACATCTCGTATGCCGTCT10.6211180124223602RNA PCR Primer, Index 39 (100% over 21bp)
GGTCTGCAGCACGCGCCTAACGGCGTGCCTCGGCATCAGCGTGCTCCGGGC10.6211180124223602No Hit
ATCCTGGGCATTTCACATCCATGAAGTAGGAATTGGGGCTCTGCACCAGGC10.6211180124223602No Hit
GTCTTTCACCATTGAATATCATGTTCACTGTGGGTTTTTCATATATGGCTT10.6211180124223602No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAAGAAAGA10.6211180124223602Illumina PCR Primer Index 8 (96% over 27bp)
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT10.6211180124223602No Hit
TTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCGCGGAA10.6211180124223602No Hit
CCAGACACTTTAAATTCCTCTCAAGAGAAATTATTTGGACCTGTCAAATCT10.6211180124223602No Hit
GAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAATCCCC10.6211180124223602No Hit
CTGTCAAGGATCTTAAGAGAGGGTACGTCGCATCCAACTCAAAGGATGACC10.6211180124223602No Hit
GAGTACGGGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGC10.6211180124223602No Hit
GAGAAGTATTTCCCCGCGGATGAGAAGCCGAGGACCACGACAATGGCTGAA10.6211180124223602No Hit
GCAGAGTACGGGGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAG10.6211180124223602No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGAAAAAA10.6211180124223602Illumina PCR Primer Index 8 (96% over 27bp)
ATAAATATCCGTAAACAGCATCTGAGCATTAGTCTTTAACCTGTCTCTTAT10.6211180124223602No Hit
CATATACACCATGGAATACTATGCAGCCATAAAAAAGGATAATTTCATGTC10.6211180124223602No Hit
GTGTATACATAAAACCCTGGGTACATATTGTTGTGGTAATAGACTGTCTCT10.6211180124223602No Hit
CATCTTGATAGAGAACAAATTTGCCTATAAGTATTACCTTCCAGAAACTCT10.6211180124223602No Hit
CAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGG10.6211180124223602No Hit
GATTCATGGAGCTAATGGCTATCTGATTGACCAGTTCATGAAGGATACGGT10.6211180124223602No Hit
CTTATACACATCTCCGAGCCCACGACTACCGCTGATCTCGTATGCCGTCTT10.6211180124223602TruSeq Adapter, Index 10 (95% over 21bp)
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTC10.6211180124223602No Hit
GCAACATGGTGCCCACCTTGCCGTTTCATTGCACCCGTCTTTGCTGACTTA10.6211180124223602No Hit
TTTTTGCGTTGATACCACCCGTACTCTGCGTTGATACCCTGTCTCTTATAC10.6211180124223602No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTCTGTCTCCTATAC10.6211180124223602No Hit
ATTTGATGTTACCTGATGCTTAGAGGCTTTTCCTGGAAGCAGGGCATTTGT10.6211180124223602No Hit
GTACTCTGCGTTGATACCACTGCCCCGTACTCTGCGTTGATACCTGTCTCT10.6211180124223602No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGATAGTCCTCTC10.6211180124223602RNA PCR Primer, Index 36 (96% over 29bp)
GGAACAACCCAGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGGAA10.6211180124223602No Hit
CTTCATGACGGACGTTAGCACCCGCCGTGTGTCTCCCGTGATAACATTCTC10.6211180124223602No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA10.6211180124223602No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATCCCCCTCCTCCCC10.6211180124223602No Hit
ATATTGCAACTTCTATGAACTCACTGCCTTTACGTTTTGTCAACACACATT10.6211180124223602No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAGTACCTGTCTC10.6211180124223602No Hit
CTCTATGAAAGTTTCCTAAATCTGCGGATGTATGTATAATCTGTCTCTTAT10.6211180124223602No Hit
GTACCAGGCCCACCATACCCACCGCCAAGTCCACCGTAGACGCCAGTACCC10.6211180124223602No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATATACACATCT10.6211180124223602Illumina PCR Primer Index 8 (96% over 27bp)
GCAGACCACAGGGCCACCAGAATCACCCTGACATGAATCCTTGCCTCCCTC10.6211180124223602No Hit
GTCTAAAATCACACAAATTAAAAGTGTTGGAGATAGAATGATAAACACAGT10.6211180124223602No Hit
CTTTTGAATGAAAGTTTTGTTTCTTAAATCTCAAAAACAGCAGAAAGCATA10.6211180124223602No Hit
TCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCGCCTCTGAC10.6211180124223602No Hit
ACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTT10.6211180124223602No Hit
ATCAAGGAAGGTGCTTTGGGAAACATTCCAATCATTGCTTTCTGTGACACA10.6211180124223602No Hit
TTCTAAAGAAGAGCAGAGGTTAGAAGTCAAAGAACTGTCTCTTATACACAT10.6211180124223602No Hit
AGTGGATGGTATTAATAGCAGAGTAGACACTGCAAAAGAACTTGAAGCTGT10.6211180124223602No Hit
CTTCTCCTATGCCGATACTCAGAGACACCGTCTTGGACCAAACTACCTTCA10.6211180124223602No Hit
GTTTTATGCCCTACGTTGTTCCTATTGTTTTATTGTATCACTGAGTTCTTA10.6211180124223602No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACATACGCATCC10.6211180124223602RNA PCR Primer, Index 27 (96% over 28bp)
GTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTC10.6211180124223602No Hit
GGCTATAGAACTCACCCTATCTTAGGTTATTCTAAATTCCACTCAGGTGTA10.6211180124223602No Hit
TTATACATCAAAAAGCTTTTGAAGTTCACCAGACTGTCCATTAATCTGTCT10.6211180124223602No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGAGACAA10.6211180124223602Illumina PCR Primer Index 5 (96% over 28bp)
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAA10.6211180124223602RNA PCR Primer, Index 27 (96% over 28bp)
TCACTGGAGAACCTACCTCCTTGGACAAATTATCCACCAGTGTCTTAATGT10.6211180124223602No Hit
TCCTACCCCCGTCCTCTTCGAGACCCCAGTCGTGATGTGGAGGAAGAGCCA10.6211180124223602No Hit
AAGTATATGCTGTTTTCCAAAGCAGGACATACTTCCCAAATACAGTTTCAA10.6211180124223602No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATACAGAAC10.6211180124223602RNA PCR Primer, Index 27 (96% over 28bp)
TCTAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACAC10.6211180124223602No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers