FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_83.35100000044da3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_83.35100000044da3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC32.608695652173913No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT21.7391304347826086No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA10.8695652173913043No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGCCTCC10.8695652173913043No Hit
GGCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGG10.8695652173913043No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGTTACTGCTGTCTCTTATA10.8695652173913043No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTCGCCTTTCCCCCCGGGT10.8695652173913043No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTCTGTCTC10.8695652173913043No Hit
ACACTGGACACTTAATAGTTTCCATGAAGAATTCTAAAAAGGGAGACTCAG10.8695652173913043No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATACAACTAT10.8695652173913043Illumina PCR Primer Index 8 (96% over 27bp)
GTTAAAAAATAGTAGAGGGATGATGCTAATAATTAGGCTGTGGGTGGTTGT10.8695652173913043No Hit
CAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTGAGACCTGTCT10.8695652173913043No Hit
CTATTTGTGTCAAAGAGCAAAAAGAAATGTCATGTGAACTTAGCTGTCTCT10.8695652173913043No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGG10.8695652173913043No Hit
GAGTACGGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTG10.8695652173913043No Hit
ATTGTGGATTGGATGATGGAAAAACCAGCAAGCCCCTCTTCTTTCAGTCTG10.8695652173913043No Hit
GTATCAACGCAGAGTACGGGAACGTCGTGACACAGTTCGAACCCTGTCTCT10.8695652173913043No Hit
ATAGCAGAGAGGAAAACTGAAGCAGAAGAGTTGAAAGATAAAGTTAGAAGC10.8695652173913043No Hit
ACAGTAACCTTTCCAGATGGTTCCTACTTAAATGATTTCATAAGGAAAACC10.8695652173913043No Hit
CTAATGGGTTGTCTAAATTGTAAGCCATATCAAATAAAATCATACACAAAG10.8695652173913043No Hit
ACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATT10.8695652173913043No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAATATAC10.8695652173913043Illumina PCR Primer Index 8 (96% over 27bp)
GTCTTGAACTTTGCCTTCAAAGATTACTTCAAAAGACTTTTCAACTTTAAG10.8695652173913043No Hit
TAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCTGTCTCTTATACACATC10.8695652173913043No Hit
CTAGTAGATAACATCTGCAATCTCTTTTCTGACTTCCTCAACAAATCTGTG10.8695652173913043No Hit
GACCTGATGCGGTTATGAGTACGACCGGGCGTGAGCGGCACTCGGTCCTCC10.8695652173913043No Hit
GAGTGTCTGAATGCATTAAAAAAAGAGAGACTCATCTATATGCTGTCTCTT10.8695652173913043No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.8695652173913043No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGGCT10.8695652173913043No Hit
GATTGTAGATATTAGGCTGTTAATTGTCAGTCTGTCTCTTATACACATCTC10.8695652173913043No Hit
CCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGT10.8695652173913043No Hit
ACCCGCGAGGGATCCCGCCAATCAGCTTCCTTGCGCCTTACGGGTTTACTC10.8695652173913043No Hit
ATCCGAGCTTATCACAAGCAAAACATCGCACAACTGGTCTCTGTGATCGTC10.8695652173913043No Hit
CACTAAACCTCTAAAAACCAGCCTTCAACTTCTTCCTTTATGATGTCACAT10.8695652173913043No Hit
GCTTCCCGTACTCTGCGTTGATACCTCTGCTTCCCGTACTCTGCGTTGATA10.8695652173913043No Hit
TTATGTCGTTGCACGATAAGATTGTTGAGGAATCTAAGAGAAGAGATAGGA10.8695652173913043No Hit
TCCTCCATCATGTCTGGAGTTACGCTGTTCTTTATGTATTGAGAGAACTGT10.8695652173913043No Hit
ATCTTAAGACTGTAGGAATAAACTATTTCATCTGTTCCTGTCTCTTATACA10.8695652173913043No Hit
GTACTTTTTTTTTTTTTTTTTTTTTGCGTTGAAACCCCTCCCCTTACCTGT10.8695652173913043No Hit
GGAGATAAAGAGCATAAATGTAACACAGATTATTCAGCCTGTCTCTTATAC10.8695652173913043No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAACCGGTC10.8695652173913043Illumina PCR Primer Index 8 (96% over 27bp)
GTTCCAATCTTTGTCTTGTAAGTAGCACCTGACTACGGTACTCGATAAGGT10.8695652173913043No Hit
TCTTCAGGAAGATCAGGAGCAAGTCCCTTAGACTTAAGAACTGTCTCTTAT10.8695652173913043No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAG10.8695652173913043No Hit
CATTGAGCTCTTCATTTCTTTAGAGCATAAACAGGCATCAAAAGAGGTTTT10.8695652173913043No Hit
GTCTAAGAAGAAGGCTTTCACTGGGTACGCTAAGCCTGTCTCTTATACACA10.8695652173913043No Hit
ACTTTACACTCTGAAAGATACCAGCCTTCCTCATCTCCTCAAAATCTTTCA10.8695652173913043No Hit
GTTGTAGACGGCAAGAAAACATTTTACAGAATCAACATCAGATGCTGTCTC10.8695652173913043No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACACGCAAGC10.8695652173913043Illumina PCR Primer Index 8 (96% over 27bp)
GCCTCCATCCCCAACAATGACAAGCTTGAAGCTGGGATAATCTACGGTTTG10.8695652173913043No Hit
TATCAACGCAGAGTACTTTTTTTTCTAAGCAGTGGTATCAACGCAGAGTAC10.8695652173913043No Hit
AAGTACTACATTCTTCTTGTACTCACTCTCCTGACTAAGGGCAATTGCTAG10.8695652173913043No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACTGTCTCTT10.8695652173913043Illumina PCR Primer Index 8 (96% over 27bp)
TTCTATATCTTCTGTTCCAGTTTTGATAGCTGTCTCTTATACACATCTCCG10.8695652173913043No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGAAACG10.8695652173913043Illumina PCR Primer Index 8 (96% over 27bp)
AACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTTATAC10.8695652173913043No Hit
GGTTTACATTACAGGTACCTAATTTCAAAGCTACCATAAACAGTAAAGCCT10.8695652173913043No Hit
AGATAAAATACTTGCAAATCCATATACAGTTGGCCCTTGAACAATGCAAAT10.8695652173913043No Hit
GAATGATGATCAAGCACTCTCCTGCGATAAGAACGGAAATAGGCTTTTCCT10.8695652173913043No Hit
GTCATCTCATTATCTAAGAGGAAACCAACGTACTTGAAACTGAGATTGATA10.8695652173913043No Hit
ACGTTGTCTCTGTCAAAGTATGCGTACCTTGAATGGTACACATAGGAGACT10.8695652173913043No Hit
AGACTTGTAGTCAACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACT10.8695652173913043No Hit
TCCTTCCCATATGGTCCTTCCCTATTACTTCTGTCTCTTATACACATCTCC10.8695652173913043No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTATTTTTCTTTTATATAAAA10.8695652173913043No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC10.8695652173913043No Hit
GGGAAACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCACAGGCTA10.8695652173913043No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAGAAAAAAAGTACCTTTCTCT10.8695652173913043No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACGATTAACGAC10.8695652173913043RNA PCR Primer, Index 27 (96% over 28bp)
TCTTATACCACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCG10.8695652173913043No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAGCATTATCTCGTATGCCGTC10.8695652173913043No Hit
GTTTTGTACAAGTTGCTCAAGATTGTACACCTTATTCAAATAGAATCCCGT10.8695652173913043No Hit
GTATCAACGCAGAGTACTTTCTCTGTCTCCTATACACATCTCCGAGCCCAC10.8695652173913043No Hit
AGCCAAGATTTTCGATTGATAAGCAGAGGTGAAAAAATCCACATTAAAACA10.8695652173913043No Hit
CTTATACACATCTCCGACGAGACAATGCGTTATCTCGTATGCCGTCTTCTG10.8695652173913043RNA PCR Primer, Index 31 (95% over 24bp)
ATCTTTGGGATCTAATTTCTTCAACCAATTTACTTTAGGGTCCTTTTTACC10.8695652173913043No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGCGTGATCTCGTATGCCGTC10.8695652173913043No Hit
TCCTTGTGCTTGATGATATTATGGATAACTCTGTCACCTGTCTCTTATACA10.8695652173913043No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTCATTCATACCACTCCCCCT10.8695652173913043No Hit
GTGAAGAAATCCATGTTTCTTTGTTTCAAAGGATAAGTTTTTTCTTGTTCT10.8695652173913043No Hit
GTAAAGGATTCTCTTTTTTAACATCCTGCTGTGTTACATCTGTCTCTTATA10.8695652173913043No Hit
GTATCAACGCAGAGTACTTTTTTTCTGTCTCTTATACACATCTCCGAGCCC10.8695652173913043No Hit
GTTAAAAAGGCACACACATTTATATTCAAAAACTAAGAACCTGTCTCTTAT10.8695652173913043No Hit
ATTTATACTCGTTCCAAGGCTTCTAACATGATGATACTATTTCCTCGTATT10.8695652173913043No Hit
GCCTCACAATGTATTTCCTTTTGTAAGATGTTTTCTCAGTTCTTCATATAC10.8695652173913043No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACAATACC10.8695652173913043Illumina PCR Primer Index 8 (96% over 27bp)
CCATTATGTAATTGCCTTCTTTGTCTCTTTTGATCTTTGTTGGTTTAAAGT10.8695652173913043No Hit
ACATTTGATATTGTGCAATACATGGTACAAACGTACAACCAAATCGCAGAC10.8695652173913043No Hit
CTTGCAGACTCTTGTTGTTGTTGTTTGCTTCTTGTTGTTGTTTGACTCATT10.8695652173913043No Hit
CCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTCTGT10.8695652173913043No Hit
GAATAGAATCATTGAATGAAATCAAATGGAATTATCATCAAATGGAATTGA10.8695652173913043No Hit
TTTAACAATTGTATCCCGATCGTCAGCCATCTTTCTGGGTTTATTTCTGTC10.8695652173913043No Hit
GATTAAATGAGAACATGGATGAGTGGACTTCATATTATGACTAGCTCTGTC10.8695652173913043No Hit
GGCCGGGGGACGGACTGGGAACGGCTCTCTCGGGAGCTTTCCCCTGTCTCT10.8695652173913043No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACTGTCTTCCTC10.8695652173913043RNA PCR Primer, Index 27 (96% over 28bp)
GTATACATGTTACATTCAGCAAGGCTAGATTACAGATTATCATAGCTGTCT10.8695652173913043No Hit
GATTTATTATATGGTTGTTACACTCGGCCACAAATAAACCTGTCTCTTATA10.8695652173913043No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCA10.8695652173913043No Hit
GAGTATCAGAACTGAAGTATCCTTCTCATTCCGGTGAAGAAGAATTATAAT10.8695652173913043No Hit
TTCCACGCCTGAATGAGGATATAAACGGAGAAAAGATACCTGATAAAACAC10.8695652173913043No Hit
TTTTTTTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCT10.8695652173913043No Hit
CCTCATCTCTAACTATTAAAATGACCCAGCCAAATGTTATCAAAACAAACA10.8695652173913043No Hit
TCTTATACACATCTCCGAGGCCACGAGACTGATTACAGTCTCGTATGCCGT10.8695652173913043No Hit
ACACTGTGCCTTGTAAGGTTACTGGTAAATGTGGTTCTGTTCTGTCTCTTA10.8695652173913043No Hit
ACCCGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCCG10.8695652173913043No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACACATCTACC10.8695652173913043Illumina PCR Primer Index 8 (96% over 27bp)
GATGCATTTGTATCTACAGTTGCATACTGTGAAGATCTGTCTCTTATACAC10.8695652173913043No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTGTCTCTTATA10.8695652173913043No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTCCCCGC10.8695652173913043No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC10.8695652173913043No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC10.8695652173913043No Hit
GGGCTGTTCTTAAGAAGAATCCATTGAAGAACCTTAATGTGATGTTCAAGT10.8695652173913043No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC10.8695652173913043No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers