FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_82.35100000044d89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_82.35100000044d89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC109.00900900900901No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGT21.8018018018018018No Hit
ATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTCTTC21.8018018018018018Illumina PCR Primer Index 5 (95% over 21bp)
ACTTATATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGACTCCGA10.9009009009009009No Hit
ATCTTTCTCCTTGTTTTTTTGCTCCAAGAAGTTCTTGAGCTGTCTCTTATA10.9009009009009009No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAAAAAAA10.9009009009009009RNA PCR Primer, Index 27 (96% over 28bp)
GGTTGAGCCCGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCT10.9009009009009009No Hit
CCACTAACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGC10.9009009009009009No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCATGATCTCGTATGCCGTC10.9009009009009009No Hit
CTCACCCTCTCTGGCGCCCCTTTCCAGGGAACTTAGGCCCGGTCCGTCGCT10.9009009009009009No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC10.9009009009009009No Hit
CTTATACACATCTCGCCCACGAGACTACCGCTGATCTCGTATGCCGTCTTC10.9009009009009009TruSeq Adapter, Index 10 (95% over 22bp)
CTTTTGTTACAGAACCAGACAAAATGGGGACTGTCTCTTATACACATCTCC10.9009009009009009No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGAAACAACGC10.9009009009009009RNA PCR Primer, Index 27 (96% over 28bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTGATAC10.9009009009009009No Hit
AACCAAACAGAGGAAATTTCTTGAATCTGTGGAGCTTCAAATCCTGTCTCT10.9009009009009009No Hit
GCATGTGGTTGGACCTGATGATGACATCGAGGCAATTAAGGAGTCGGCTAT10.9009009009009009No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTATA10.9009009009009009No Hit
GGTCAGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAACCCTGTCTCCTG10.9009009009009009No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCCATGATCTCGTATGCCGTC10.9009009009009009No Hit
CTGGAAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGACCCTGT10.9009009009009009No Hit
GACGAAGCTGAGGCGCGTGAACCGGTGGATTTGAGTCCAGAGAAAGCTGGT10.9009009009009009No Hit
ACCTAGAACCCACAAAGGGTGTTGGCCGATTAAGATAGCAGGACGGTGGTC10.9009009009009009No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC10.9009009009009009No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATTCTCGTATGCC10.9009009009009009No Hit
AATGAAAGTTGACTAAATAACGTCGTAATCTGCATTCCAATTCGAATACCT10.9009009009009009No Hit
GATTACAGCTCCCTCGCCGAGCACCACTCCCTCATATCTTGCTGGCAGAGC10.9009009009009009No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATA10.9009009009009009No Hit
GGTATGTGCTTTCTCGTGTTACATCGCGCCATCATTGGTATATGGTTAGTG10.9009009009009009No Hit
GTTAAACACATTCGTGTATTGTTTAACGTTTTTAACAGCTGTCTCTTATAC10.9009009009009009No Hit
GTACATGCTATCCTTACTTACAATGGAACCAACCATTTGATCTGTCTCTTA10.9009009009009009No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGCAACCCCAT10.9009009009009009RNA PCR Primer, Index 27 (96% over 28bp)
AACGTAGACATGTGATAACACAAGTAGATGTATTGCAAAATCCTCCATATC10.9009009009009009No Hit
GTGCTCTACCCCCGGAGATGAATTCACGAGGCGCTACCTAAATAGCTTTCG10.9009009009009009No Hit
GCCGGGTTCCTCGGCGGCATCGTCAAGCGGGCCGGGCTCGCCGACTTCTCG10.9009009009009009No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGACTGTCTCTTATACACAT10.9009009009009009No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACCATGATCTCGTATGCCGTC10.9009009009009009No Hit
TTTTTTGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCT10.9009009009009009No Hit
CCTTGTAGGCCCAGTGCGCGGCCACGCCGCTTTCGGCGTGCTCGTGCATGG10.9009009009009009No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTGA10.9009009009009009No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAACTTTATCTCGTATGCCGTC10.9009009009009009No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACGAAACA10.9009009009009009Illumina PCR Primer Index 8 (96% over 27bp)
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAAAAACCA10.9009009009009009Illumina PCR Primer Index 8 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACTCAGCATGATCTCGTATGCCGTC10.9009009009009009No Hit
GTCGTAAGGGCTGAGGATTTTTGGTGCGCACGCTCCTGCTCCTGACTCATC10.9009009009009009No Hit
GGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTG10.9009009009009009No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTC10.9009009009009009No Hit
ACTTATGACTACAAATTTTAAAAAGACTCATAACAAAAGCAAAAGCATAAA10.9009009009009009No Hit
TCTTGAAACACGGACCAAGGAGTCTGACATGTGTGCGAGTCAACGGGTGAG10.9009009009009009No Hit
GGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACG10.9009009009009009No Hit
CATCAAAAGAGAGCATCTGGAAGAACAGCCAGCTTGGAAGTTTTACAGCAA10.9009009009009009No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACG10.9009009009009009No Hit
GAAATCACAGTCATTATTTAGTTGCCATTGTCCTGTTACTGTCTCTTATAC10.9009009009009009No Hit
AACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTTATAC10.9009009009009009No Hit
AGCCAACTAAGTGCTATATATTAGCAGCATGACTTGTCTAACAACAAGCAA10.9009009009009009No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT10.9009009009009009No Hit
TCCCATGTGTGTATATCAAATATGATAATGTGGAAGAGCTGTCTCTTATAC10.9009009009009009No Hit
CTTATACACATCTCCGAGCCCACGGAGACTACCGCTGATCTCGTATGCCGT10.9009009009009009No Hit
ATCAAAAGACAGATTCAAATCTGTTTGGTGAATTCACACATCTCAAAGACG10.9009009009009009No Hit
GATAAGGTTTAGTGGACTTCTCACGACGTCGCGGGCGGCGCTCTACCCACG10.9009009009009009No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT10.9009009009009009No Hit
TGTCAATGGGATCCATTCTATGCTCCAGAGCTTCGAGCCTATTCATGGTGT10.9009009009009009No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA10.9009009009009009No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCG10.9009009009009009No Hit
CACACGTACAAGGGAAACAAAAGTGCTGAGAAGGCACTCATTGCAGCAGAG10.9009009009009009No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAGAAAAAAAAAAAAAAAAAA10.9009009009009009No Hit
TATCAACGCAGAGTACGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCC10.9009009009009009No Hit
GGACAGAAGTAGGTTTTTGAATGTAACAAGATAAGTCAACTTGAGTTGTAA10.9009009009009009No Hit
GTATTTTCCATCCTTTACATCCTTCTGTCTGTTCAAGAACCAGTCTGGGAT10.9009009009009009No Hit
GTCACACGGAATCGAGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTG10.9009009009009009No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT10.9009009009009009No Hit
AAGTTTTCTCTGGGCTAACTTGGTAAACATTGTCTACTGCTGTCTCTGTAT10.9009009009009009No Hit
ACCCAGACCACCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGGAAAAAAAA10.9009009009009009No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCCTGATCTCGTATGCCGTC10.9009009009009009No Hit
GTATCAACGCAGAGTACGGGCAGAGTACTTTTTTTTTTTTTTTTGGTTTTA10.9009009009009009No Hit
AGTTTCTACAAATTGCTCAATCCAATGGATTCATGTATCCCTGTCTCTTAT10.9009009009009009No Hit
TTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC10.9009009009009009No Hit
CCGCCAATCAGCTTCCTTGCGCCTTACGGGTTTACTCACCCGTTGACTCGC10.9009009009009009No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACATATCAG10.9009009009009009Illumina PCR Primer Index 8 (96% over 27bp)
GTCCACTCAAAATTCATGGGCCCTTCCATGGTACAGGATGCTTCCTGAGAA10.9009009009009009No Hit
ATACTATTGAGAGCTTGAGATGGAGATAGCAGGATATTTTGATTGCAGATA10.9009009009009009No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACACCCACCCTT10.9009009009009009Illumina PCR Primer Index 8 (96% over 27bp)
GTATAGATGAGATAGCGGTTGTCGAAGGACTTGATGAGTTTGTGAAGGAAT10.9009009009009009No Hit
AGCAAGAACAAATGAAGCTTCTGCTGAGTAGTCCCTCCTAGTGTTTCTGTC10.9009009009009009No Hit
GTATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGA10.9009009009009009No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTAAA10.9009009009009009No Hit
ATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA10.9009009009009009No Hit
ACTTTCTTCTTTTCTGTTGTTTGTTGACGGTATAGTTGATTTACTGTCTCT10.9009009009009009No Hit
GTCAGTCTCGCAGTCAGGCGGGCTTATGCCATTGCACTCGACGACCGATTT10.9009009009009009No Hit
TTATAATTCCAATCACTCGTTTAAAAGCGAAAGAAAAAACAATTACACCGA10.9009009009009009No Hit
CTATTCTACAATACAAAACTGGAAACAAGTGGTACAACTGTCTCTTATACA10.9009009009009009No Hit
GGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTGGAAGAC10.9009009009009009No Hit
GAGTATGTAAATTGGAAGTTAACTTATGCACGCTTAACTATCTTAACAAGC10.9009009009009009No Hit
TAAGAGAACTCGGGTGAAGGAACTAGGCAAAATGGTGCCGTAACTTCGGGA10.9009009009009009No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAATACTTGGGTTTA10.9009009009009009No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC10.9009009009009009No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCATGATCTCGTATGCCGTC10.9009009009009009No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATGGCTTCAAA10.9009009009009009RNA PCR Primer, Index 27 (96% over 28bp)
ATGCTTACCAATAGCCTTCACTCTCTCGATGTTCTTGTATCTTAAGGTTGA10.9009009009009009No Hit
CTTATCCACATCTCCGAGCCCACGAGACTCGCCATGAACCCGTATGCCGTC10.9009009009009009No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers