FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_80.35100000044d39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_80.35100000044d39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47088
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC1676835.60992184845396No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGT25675.451495073054706No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCG3630.7708970438328236No Hit
TTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTCT2950.6264865783214407No Hit
ATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTCTTC2720.5776418620455317Illumina PCR Primer Index 7 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTCTT1100.23360516479782537Illumina PCR Primer Index 7 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCCTGGACCATCTCGTATGCCGTC700.1486578321440707No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCG590.12529731566428814No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC17050.042.75559245
ATGCCGT19700.036.8899944
ATCTCGT20050.036.35824237
CATCTCG19950.036.3149336
TCTCGTA20050.036.24602538
CTCGTAT20050.036.24602539
CGAGCCC20150.036.17780715
TCCGAGC20150.036.17780713
CTCCGAG20150.036.17780712
AGCCCAC20100.036.15586517
CACGAGA20100.036.15586521
TCGTATG20100.036.15586540
ACGAGAC20050.036.1338122
CCATCTC20050.036.1338135
AGACTCT20050.036.1338125
GACCATC20050.036.1338133
GAGACTC20050.036.1338124
GACTCTG20000.036.1116526
GAGCCCA20250.036.11025616
ATCTCCG19950.036.0893710