Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_0113_pb_8.35100000044432.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 59810 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 52 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC | 8469 | 14.15983949172379 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT | 631 | 1.0550075238254473 | RNA PCR Primer, Index 44 (95% over 21bp) |
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT | 306 | 0.5116201304129744 | RNA PCR Primer, Index 44 (95% over 23bp) |
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG | 195 | 0.32603243604748366 | No Hit |
ATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTTC | 114 | 0.1906035779969905 | RNA PCR Primer, Index 37 (95% over 23bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 945 | 0.0 | 41.660473 | 45 |
ATGCCGT | 1000 | 0.0 | 39.36915 | 44 |
TCGTATG | 1015 | 0.0 | 39.00898 | 40 |
ATCTCGT | 1020 | 0.0 | 38.817757 | 37 |
GACCCTA | 1020 | 0.0 | 38.817757 | 26 |
TATCTCG | 1020 | 0.0 | 38.817757 | 36 |
TCTCGTA | 1020 | 0.0 | 38.817757 | 38 |
CCTAGAA | 1010 | 0.0 | 38.789047 | 29 |
TAGAATA | 1010 | 0.0 | 38.789047 | 31 |
GAATATC | 1010 | 0.0 | 38.789047 | 33 |
AGAATAT | 1010 | 0.0 | 38.789047 | 32 |
ACCCTAG | 1010 | 0.0 | 38.756615 | 27 |
CGTATGC | 1030 | 0.0 | 38.6593 | 41 |
CACGAGA | 1030 | 0.0 | 38.6593 | 21 |
TATGCCG | 1030 | 0.0 | 38.6593 | 43 |
CGAGCCC | 1030 | 0.0 | 38.6593 | 15 |
CTCGTAT | 1025 | 0.0 | 38.628403 | 39 |
GAGACCC | 1025 | 0.0 | 38.628403 | 24 |
GTATGCC | 1035 | 0.0 | 38.47254 | 42 |
ACGAGAC | 1035 | 0.0 | 38.47254 | 22 |