FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_8.35100000044432.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_8.35100000044432.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59810
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC846914.15983949172379No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT6311.0550075238254473RNA PCR Primer, Index 44 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT3060.5116201304129744RNA PCR Primer, Index 44 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG1950.32603243604748366No Hit
ATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTTC1140.1906035779969905RNA PCR Primer, Index 37 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC9450.041.66047345
ATGCCGT10000.039.3691544
TCGTATG10150.039.0089840
ATCTCGT10200.038.81775737
GACCCTA10200.038.81775726
TATCTCG10200.038.81775736
TCTCGTA10200.038.81775738
CCTAGAA10100.038.78904729
TAGAATA10100.038.78904731
GAATATC10100.038.78904733
AGAATAT10100.038.78904732
ACCCTAG10100.038.75661527
CGTATGC10300.038.659341
CACGAGA10300.038.659321
TATGCCG10300.038.659343
CGAGCCC10300.038.659315
CTCGTAT10250.038.62840339
GAGACCC10250.038.62840324
GTATGCC10350.038.4725442
ACGAGAC10350.038.4725422