FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_79.35100000044d2c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_79.35100000044d2c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190074
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC5022226.42234077254122No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGT172829.092248282247967No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCG18540.9754095773225165No Hit
TTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTCT9820.5166408872333933No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCG2380.12521439018487537No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC56600.043.28051445
ATGCCGT73950.033.0957144
GCCGTCT2050.032.9191345
CGTCTTC551.12160706E-732.71961645
CCGTCTT551.12160706E-732.71961644
GACCATC75700.032.36884333
GGACCAT75750.032.31777632
GAGCCCA75800.032.3176416
CCGAGCC75600.032.3138714
AGCCCAC75700.032.30089617
CGAGCCC75700.032.30089615
TGGACCA75750.032.2880731
ATCTCGT75350.032.27181237
CTCGTAT75350.032.27181239
TCTCGTA75300.032.2633738
CGAGACT75600.032.26284823
ACGAGAC75850.032.24550622
TATGCCG75900.032.2454243
CATCTCG75350.032.2419636
GCCCACG75850.032.2370218
Produced by FastQC (version 0.11.2)