FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_78.35100000044d02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_78.35100000044d02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41190
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC728217.679048312697258No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGT3860.937120660354455No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCG3840.9322651128914785No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCACG206.954201E-445.0000041
TGCCGTC8850.043.22033745
ATGCCGT9050.042.0165744
AGCCCAC9500.041.21052617
CCCACGA9500.041.21052619
GAGACTC9500.041.21052624
CTCTGGA9450.041.1904728
CACGAGA9600.041.0156221
GCCCACG9550.040.99476618
TGGACCA9350.040.90909631
GGACCAT9300.040.88709632
GAGCCCA9600.040.78124616
CGAGACT9600.040.78124623
CGAGCCC9550.040.75916315
CCGAGCC9550.040.75916314
AGACTCT9550.040.75916325
TCTGGAC9500.040.7368429
ATCTCGT9350.040.66845337
ACCATCT9250.040.6216234
ACGAGAC9700.040.59278522
Produced by FastQC (version 0.11.2)