FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_77.35100000044ce7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_77.35100000044ce7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176246
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC11193563.510661234864905No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGT137717.813510661234865No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCG46072.6139600331355037No Hit
TTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTCT6960.39490257934931855No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGGACCATCTCGTATGCCGTC3520.199720844728391No Hit
ATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTCTTC2140.12142119537464681Illumina PCR Primer Index 7 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC115450.044.2560745
CCCTGGA800.039.3720228
ATGCCGT131100.038.9215444
GCCCACG136300.037.5191718
AGCCCAC136550.037.51638417
GAGCCCA136750.037.5108716
TCCGAGC136750.037.5108713
CCGAGCC136700.037.50813314
CGAGCCC136650.037.50539415
GAGACCC900.037.4971624
CCCACGA136450.037.4944119
TCTCGTA135450.037.48885738
CCATCTC135500.037.4750235
CCACGAG136550.037.46695720
ATCTCGT135650.037.46675537
CTCGTAT135650.037.46675539
ACGAGAC136500.037.46419522
CACGAGA136700.037.4587621
ACCATCT135700.037.4529534
TATGCCG136250.037.43386543