FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_76.35100000044ccd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_76.35100000044ccd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363990
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC323498.887332069562351No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGT109243.00118135113602No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCG14680.4033077831808566No Hit
GAGTCATTGTGGTTAGGGATTAGAGTCTCAACATCTCCCAAGTAGCTCCCT8280.227478776889475No Hit
GGAAGAAGGATTTCATTGAGATACTCAAGACTCTTGAATCTGAGCTTGGAG6400.17582900629138162No Hit
AAAGAAGCAGTTGAGCAGAGAAAAGCTGAGAAGAGAGGCTTGAAATCTGAT6340.17418060935739993No Hit
GATTCAAGAGTCTTGAGTATCTCAATGAAATCCTTCTTCCCTGTCTCTTAT6160.16923541855545482No Hit
GTCATTGTGGTTAGGGATTAGAGTCTCAACATCTCCCAAGTAGCTCCCTCT5800.1593450369515646No Hit
CTCCATTTCTTCACGTTCTTCTTCATCTCTTTGCTCCTCTCTCCTTCCATC5490.1508283194593258No Hit
GGAAGAAGGATTTCATTGAGATACTCAAGACTCTTGAATCCTGTCTCTTAT5460.15000412099233495No Hit
TTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTCT4730.12994862496222426No Hit
GGTTTGTCTCCAAGCTCAGTTTCAAGAATCTTGAGTGCTTCTATATACTCT4690.1288496936729031No Hit
CTCCAAGCTCAGATTCAAGAGTCTTGAGTATCTCAATGAAATCCTTCTTCC4070.1118162586884255No Hit
AACTCAGTCTCATGATCGATATTCTCGTCTCTTATACACATCTCCGAGCCC3830.1052226709524987No Hit
GCTGTAATATCCAGTCATGGCAATGTCTACGAACCCGAAGGTATCTCCACC3740.10275007555152614No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGA207.0187915E-445.0105822
TGCCGTC32650.043.48219345
GACGGTA356.238228E-638.56989724
TAGGACG301.1386558E-437.4985121
ACGCTAG250.0020985436.0233151
TACTATA250.002102792336.0084652
TGGGGCC751.8189894E-1235.99857328
AGCTGAG504.865069E-835.99857324
ATCGCTA250.00210563135.99857310
AGCCCTC458.6809086E-734.9986116
GTGCAGA352.8059326E-432.1636731
CTAGTAC708.076313E-1032.1504174
AGCCCCC352.8172717E-432.14158245
TGGGTGG708.112693E-1032.14158226
GCCGTCT1050.032.14158245
ATGCCGT44750.031.72544
CAGCCCT501.9773142E-631.4987515
TCAGCCC501.9773142E-631.4987514
GCCCTCA501.9773142E-631.4987517
CATCTCG46200.030.43710536
Produced by FastQC (version 0.11.2)